Bunyavirus superinfection and segment reassortment in transovarially infected mosquitoes

被引:40
作者
Borucki, MK
Chandler, LJ
Parker, BM
Blair, CD
Beaty, BJ [1 ]
机构
[1] Colorado State Univ, Dept Microbiol, Arthropod Borne & Infect Dis Lab, Ft Collins, CO 80523 USA
[2] N Carolina State Univ, Dept Entomol, Raleigh, NC 27695 USA
关键词
D O I
10.1099/0022-1317-80-12-3173
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Rapid evolution of bunyaviruses may occur by RNA segment reassortment between closely related viruses. Reassortment between viruses occurs in dually infected mosquitoes when two different viruses are simultaneously ingested or when the second virus is ingested within 2 days of the first virus. By 3 days after oral infection, interference to superinfection occurs, thus limiting the potential for evolution. Aedes triseriatus mosquitoes can also be transovarially infected (TI+) with LaCrosse (LAC) virus. In these studies, the potential for oral superinfection of TI+ mosquitoes was assessed. Approximately 20% of mosquitoes TI+ with either a temperature-sensitive LAC virus or wild-type (wt) LAC virus became superinfected by ingesting blood meals containing wt LAC or snowshoe hare (SSH) viruses. LAC virus TI+ mosquitoes superinfected with SSH virus were detected by blot hybridization or RT-PCR. Viruses from these mosquitoes were plaque purified and genotyped using RT-PCR. Reassortant genomes were detected in 2.3% of the viruses genotyped, and 4.0% of the genomes tested were diploid for one genome segment.
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页码:3173 / 3179
页数:7
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