Genetic organization of a plasmid from an industrial wastewater bioreactor

被引：9

作者：

Bramucci, Michael ^{[1
]}

Chen, Mario ^{[1
]}

Nagarajan, Vasantha ^{[1
]}

机构：

[1] DuPont Co Inc, Cent Res & Dev, Wilmington, DE 19880 USA

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2006年 / 71卷 / 01期

关键词：

D O I：

10.1007/s00253-005-0119-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Pseudomonas strain CT14 was isolated from activated sludge. Strain CT14 contained a 55, 216 bp plasmid that was characterized by sequence analysis. The plasmid had a modular structure with 51 open reading frames (ORFs) that were distributed between two clearly demarcated domains. Domain I primarily contained genes for plasmid-related functions and a novel origin of replication. Domain II bore evidence of extensive transposition and recombination. Domain II contained several genes from a meta-cleavage pathway for aromatic rings. These genes appeared to have been recruited from different hosts. This observation suggests that sequencing pCT14 may have revealed an intermediate stage in the evolution of a new assemblage of meta-cleavage pathway genes.

引用

页码：67 / 74

页数：8

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