Characterization of the gtfB and gtfC promoters from Streptococcus mutans GS-5

被引:35
作者
Goodman, SD [1 ]
Gao, Q [1 ]
机构
[1] Univ So Calif, Sch Dent, Dept Basic Sci, Los Angeles, CA 90089 USA
关键词
D O I
10.1006/plas.1999.1444
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
We have characterized the promoter regions of the gtfB and gtfC genes from Streptococcus mutans GS-5. Using a plasmid-based reporter system, we discovered that the gtfC promoter is an order of magnitude stronger than the gtfB promoter. In addition, we found that a variety of growth conditions failed to affect or discriminate between the expression of the two promoters. Only during the various phases of growth could we demonstrate significant changes in expression. We conclude that gtfB and gtfC promoters are coordinately expressed, (C) 2000 Academic Press.
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页码:85 / 98
页数:14
相关论文
共 34 条
[21]   Identification of a homolog of CcpA catabolite repressor protein in Streptococcus mutans [J].
Simpson, CL ;
Russell, RRB .
INFECTION AND IMMUNITY, 1998, 66 (05) :2085-2092
[22]   PRIMER EXTENSION ANALYSIS OF STREPTOCOCCUS-MUTANS PROMOTER STRUCTURES [J].
SMORAWINSKA, M ;
KURAMITSU, HK .
ORAL MICROBIOLOGY AND IMMUNOLOGY, 1995, 10 (03) :188-192
[23]   Regulated expression of the Streptococcus mutans dlt genes correlates with intracellular polysaccharide accumulation [J].
Spatafora, GA ;
Sheets, M ;
June, R ;
Luyimbazi, D ;
Howard, K ;
Hulbert, R ;
Barnard, D ;
El Janne, M ;
Hudson, MC .
JOURNAL OF BACTERIOLOGY, 1999, 181 (08) :2363-2372
[24]   Rgg is a positive transcriptional regulator of the Streptococcus gordonii gtfG gene [J].
Sulavik, MC ;
Clewell, DB .
JOURNAL OF BACTERIOLOGY, 1996, 178 (19) :5826-5830
[25]   Acid tolerance response and survival by oral bacteria [J].
Svensater, G ;
Larsson, UB ;
Greif, ECG ;
Cvitkovitch, DG ;
Hamilton, IR .
ORAL MICROBIOLOGY AND IMMUNOLOGY, 1997, 12 (05) :266-273
[26]  
TERECKYJ B, 1975, INFECT IMMUN, V11, P649
[27]   Effects of surfactants on glucosyltransferase production and in vitro sucrose-dependent colonization by Streptococcus mutans [J].
Tomita, Y ;
Watanabe, T ;
Takeuchi, T ;
Nanbu, A ;
Shinozaki, N ;
Ikemi, T ;
Fukushima, K .
ARCHIVES OF ORAL BIOLOGY, 1998, 43 (09) :735-740
[28]   The role of the Streptococcus mutans glucosyltransferases in the sucrose-dependent attachment to smooth surfaces: essential role of the GtfC enzyme [J].
Tsumori, H ;
Kuramitsu, H .
ORAL MICROBIOLOGY AND IMMUNOLOGY, 1997, 12 (05) :274-280
[29]   MOLECULAR-BASIS FOR THE SPONTANEOUS GENERATION OF COLONIZATION-DEFECTIVE MUTANTS OF STREPTOCOCCUS-MUTANS [J].
UEDA, S ;
KURAMITSU, HK .
MOLECULAR MICROBIOLOGY, 1988, 2 (01) :135-140
[30]   SEQUENCE-ANALYSIS OF THE GTFC GENE FROM STREPTOCOCCUS-MUTANS-GS-5 [J].
UEDA, S ;
SHIROZA, T ;
KURAMITSU, HK .
GENE, 1988, 69 (01) :101-109