A molecular basis for phosphorylation-dependent SUMO conjugation by the E2 UBC9

被引:68
作者
Mohideen, Firaz [1 ]
Capili, Allan D. [1 ]
Bilimoria, Parizad M. [2 ]
Yamada, Tomoko [2 ]
Bonni, Azad [2 ]
Lima, Christopher D. [1 ]
机构
[1] Sloan Kettering Inst, Program Struct Biol, New York, NY USA
[2] Harvard Univ, Sch Med, Dept Pathol, Boston, MA 02115 USA
基金
美国国家卫生研究院;
关键词
ACTIVATED RECEPTOR-GAMMA; UBIQUITIN-LIKE PROTEINS; TRANSCRIPTIONAL ACTIVITY; DENDRITIC MORPHOGENESIS; MECHANISTIC INSIGHTS; STRUCTURAL BASIS; E3; LIGASE; MEF2; SUMOYLATION; SYSTEM;
D O I
10.1038/nsmb.1648
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Phosphorylation and small ubiquitin-like modifier (SUMO) conjugation contribute to the spatial and temporal regulation of substrates containing phosphorylation-dependent SUMO consensus motifs (PDSMs). Myocyte-enhancement factor 2 (MEF2) is a transcription factor and PDSM substrate whose modification by SUMO drives postsynaptic dendritic differentiation. NMR analysis revealed that the human SUMO E2 interacted with model substrates for phosphorylated and nonphosphorylated MEF2 in similar extended conformations. Mutational and biochemical analysis identified a basic E2 surface that enhanced SUMO conjugation to phosphorylated PDSM substrates MEF2 and heat-shock transcription factor 1 (HSF1), but not to nonphosphorylated MEF2 or HSF1, nor the non-PDSM substrate p53. Mutant ubiquitin-conjugating enzyme UBC9 isoforms defective in promoting SUMO conjugation to phosphorylated MEF2 in vitro and in vivo also impair postsynaptic differentiation in organotypic cerebellar slices. These data support an E2-dependent mechanism that underlies phosphorylation-dependent SUMO conjugation in pathways that range from the heat-shock response to nuclear hormone signaling to brain development.
引用
收藏
页码:945 / U68
页数:9
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