The serine 2481-autophosphorylated form of mammalian Target Of Rapamycin (mTOR) is localized to midzone and midbody in dividing cancer cells

被引:27
作者
Vazquez-Martin, Alejandro [1 ,2 ]
Oliveras-Ferraros, Cristina [1 ,2 ]
Bernado, Luis [2 ,3 ]
Lopez-Bonet, Eugeni [2 ,3 ]
Menendez, Javier A. [1 ,2 ]
机构
[1] Dr Josep Trueta Univ Hosp Girona, Catalan Inst Oncol Girona ICO Girona, E-17007 Girona, Catalonia, Spain
[2] Girona Biomed Res Inst IdiBGi, Girona, Catalonia, Spain
[3] Dr Josep Trueta Univ Hosp Girona, Dept Pathol, E-17007 Girona, Catalonia, Spain
关键词
mTOR; Cell cycle; Mitosis; Cytokinesis; Chromosomal passenger protein; Cancer; CHROMOSOMAL PASSENGERS; CYTOKINESIS; COMPLEX; GROWTH;
D O I
10.1016/j.bbrc.2009.01.153
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
070307 [化学生物学]; 071010 [生物化学与分子生物学];
摘要
Using a high-resolution, automated confocal high-content imaging system, we investigated the sub-cellular localization of the Serine 2481-autophosphorylated form of mTOR (PP-mTOR(Ser2481)) during mitosis and cytokinesis in human cancer cells. PP-mTOR(Ser2481) exhibited a punctate nuclear distribution in interphase cancer cells, with the number of PP-mTOR(Ser2481) nuclear speckles positively relating with the proliferative capacity of cancer cells. PP-mTOR(Ser2481) expression dynamically rearranged within the cytoplasm in a close association near and between separating chromosomes during early stages of mitosis. Towards the end of anaphase and in telophase, PP-mTOR(Ser2481) drastically focused on the midzone and ultimately in the centre of the midbody at the presumptive cleavage furrow. In cells at cytokinesis, PP-mTOR(Ser2481) appeared as a doublet facing each other at the apical ends of two daughter cells. Three-dimensional analysis confirmed that PP-mTOR(Ser2481) positioned at a ring structure wrapped round by microtubule bundles to connect daughter cells. These results reveal for the first time that PP-mTOR(Ser2481) may be unexpectedly involved in the terminal stages of cytokinesis. (C) 2009 Elsevier Inc. All rights reserved.
引用
收藏
页码:638 / 643
页数:6
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