The development of cowpea mosaic virus as a potential source of novel vaccines

被引：53

作者：

Porta, C

Spall, VE

Lin, TW

Johnson, JE

Lomonossoff, GP

机构：

[1] JOHN INNES CTR, DEPT VIRUS RES, NORWICH NR4 7UH, NORFOLK, ENGLAND

[2] PURDUE UNIV, DEPT BIOL SCI, W LAFAYETTE, IN 47907 USA

[3] Scripps Res Inst, DEPT MOL BIOL, LA JOLLA, CA 92037 USA

来源：

INTERVIROLOGY | 1996年 / 39卷 / 1-2期

关键词：

cowpea mosaic virus; cowpea plants; rhinovirus; 14; human; human immunodeficiency virus type 1; virus crystals; X-ray diffraction;

D O I：

10.1159/000150478

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Epitopes from human rhinovirus 14 (HRV-14) and human immunodeficiency virus type (HIV-1) have been expressed on the surface of particles of the plant virus, cowpea mosaic virus (CPMV). The chimaeras retain their ability to grow in plants and large quantities of virions can be easily purified. Immunological studies have shown that purified particles have the antigenic properties of the insert, and, in the case of the HIV-1 chimaera, can elicit the production of neutralising antibodies in mice. The chimaera containing the epitope from HRV-14 has been crystallised and the crystals shown to diffract to atomic resolution.

引用

收藏

页码：79 / 84

页数：6

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