Male-female communication triggers calcium signatures during fertilization in Arabidopsis

被引：140

作者：

Denninger, Philipp ^{[1
,2
,3
]}

Bleckmann, Andrea ^{[1
]}

Lausser, Andreas ^{[1
]}

Vogler, Frank ^{[1
]}

Ott, Thomas ^{[4
]}

Ehrhardt, David W. ^{[5
]}

Frommer, Wolf B. ^{[5
]}

Sprunck, Stefanie ^{[1
]}

Dresselhaus, Thomas ^{[1
]}

Grossmann, Guido ^{[2
,3
,5
]}

机构：

[1] Univ Regensburg, Biochem Zentrum Regensburg, D-93053 Regensburg, Germany

[2] Heidelberg Univ, Cell Networks Cluster Excellence, D-69120 Heidelberg, Germany

[3] Heidelberg Univ, COS, D-69120 Heidelberg, Germany

[4] Univ Munich, Inst Genet, D-82152 Martinsried, Germany

[5] Carnegie Inst Sci, Dept Plant Biol, Stanford, CA 94305 USA

来源：

NATURE COMMUNICATIONS | 2014年 / 5卷

关键词：

IN-VITRO FERTILIZATION; GENETICALLY ENCODED INDICATORS; POLLEN-TUBE RECEPTION; PLASMA-MEMBRANE; EGG ACTIVATION; TROPONIN-C; FLUORESCENT INDICATORS; CYTOPLASMIC CA2+; FLOWERING PLANTS; SYNERGID CELLS;

D O I：

10.1038/ncomms5645

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Cell-cell communication and interaction is critical during fertilization and triggers free cytosolic calcium ([Ca2+](cyto)) as a key signal for egg activation and a polyspermy block in animal oocytes. Fertilization in flowering plants is more complex, involving interaction of a pollen tube with egg adjoining synergid cells, culminating in release of two sperm cells and their fusion with the egg and central cell, respectively. Here, we report the occurrence and role of [Ca2+](cyto) signals during the entire double fertilization process in Arabidopsis. [Ca2+](cyto) oscillations are initiated in synergid cells after physical contact with the pollen tube apex. In egg and central cells, a short [Ca2+](cyto) transient is associated with pollen tube burst and sperm cell arrival. A second extended [Ca2+](cyto) transient solely in the egg cell is correlated with successful fertilization. Thus, each female cell type involved in double fertilization displays a characteristic [Ca2+](cyto) signature differing by timing and behaviour from [Ca2+](cyto) waves reported in mammals.

引用

页数：12

共 69 条

[1] Defensin-Like ZmES4 Mediates Pollen Tube Burst in Maize via Opening of the Potassium Channel KZM1 [J].

Amien, Suseno ;

Kliwer, Irina ;

Marton, Mihaela L. ;

Debener, Thomas ;

Geiger, Dietmar ;

Becker, Dirk ;

Dresselhaus, Thomas .

PLOS BIOLOGY, 2010, 8 (06)

[2]

[Anonymous], 2012, J VIS EXP

[3] A calcium influx is triggered and propagates in the zygote as a wavefront during in vitro fertilization of flowering plants [J].

Antoine, AF ;

Faure, JE ;

Cordeiro, S ;

Dumas, C ;

Rougier, M ;

Feijó, JA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (19) :10643-10648

[4] Differential contribution of cytoplasmic Ca2+ and Ca2+ influx to gamete fusion and egg activation in maize [J].

Antoine, AF ;

Faure, JE ;

Dumas, C ;

Feijó, JA .

NATURE CELL BIOLOGY, 2001, 3 (12) :1120-1123

[5] Gamete Fusion Is Required to Block Multiple Pollen Tubes from Entering an Arabidopsis Ovule [J].

Beale, Kristin M. ;

Leydon, Alexander R. ;

Johnson, Mark A. .

CURRENT BIOLOGY, 2012, 22 (12) :1090-1094

[6] ANXUR Receptor-Like Kinases Coordinate Cell Wall Integrity with Growth at the Pollen Tube Tip Via NADPH Oxidases [J].

Boisson-Dernier, Aurelien ;

Lituiev, Dmytro S. ;

Nestorova, Anna ;

Franck, Christina Maria ;

Thirugnanarajah, Sharme ;

Grossniklaus, Ueli .

PLOS BIOLOGY, 2013, 11 (11)

[7] Maternal Control of Male-Gamete Delivery in Arabidopsis Involves a Putative GPI-Anchored Protein Encoded by the LORELEI Gene [J].

Capron, Arnaud ;

Gourgues, Mathieu ;

Neiva, Lissiene S. ;

Faure, Jean-Emmanuel ;

Berger, Frederic ;

Pagnussat, Gabriela ;

Krishnan, Anjali ;

Alvarez-Mejia, Cesar ;

Vielle-Calzada, Jean-Philippe ;

Lee, Yuh-Ru ;

Liu, Bo ;

Sundaresan, Venkatesan .

PLANT CELL, 2008, 20 (11) :3038-3049

[8] Arbuscular mycorrhizal hyphopodia and germinated spore exudates trigger Ca2+ spiking in the legume and nonlegume root epidermis [J].

Chabaud, Mireille ;

Genre, Andrea ;

Sieberer, Bjoern J. ;

Faccio, Antonella ;

Fournier, Joelle ;

Novero, Mara ;

Barker, David G. ;

Bonfante, Paola .

NEW PHYTOLOGIST, 2011, 189 (01) :347-355

[9] Floral dip:: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana [J].

Clough, SJ ;

Bent, AF .

PLANT JOURNAL, 1998, 16 (06) :735-743

[10]

Digonnet C, 1997, DEVELOPMENT, V124, P2867

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