Upregulation of monocyte urokinase plasminogen activator receptor during human endotoxemia

被引：61

作者：

Dekkers, PEP ^{[1
]}

Ten Hove, T ^{[1
]}

Te Velde, AA ^{[1
]}

Van Deventer, SJH ^{[1
]}

Van der Poll, T ^{[1
]}

机构：

[1] Univ Amsterdam, Acad Med Ctr, Lab Expt Internal Med, NL-1105 AZ Amsterdam, Netherlands

来源：

INFECTION AND IMMUNITY | 2000年 / 68卷 / 04期

关键词：

D O I：

10.1128/IAI.68.4.2156-2160.2000

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The receptor for urokinase-type plasminogen activator (uPAR) (CD87) plays an important role in leukocyte adhesion and migration. To assess the effect of endotoxin on cellular uPAR, uPAR expression was determined on leukocytes bg fluorescence-activated cell sorter analysis in seven healthy subjects following intravenous injection of endotoxin (Iot G; 4 ng/kg). Endotoxin induced a transient increase in uPAR expression on monocytes, reaching a 92% +/- 46% increase over baseline expression after 6 h (P < 0.05). Endotoxin did not influence uPAR expression on granulocytes, while uPAR remained undetectable on lymphocytes. Endotoxin also increased soluble uPAR levels in plasma (P < 0.05). Stimulation of human whole blood with endotoxin or gram-positive stimuli in vitro also resulted in an upregulation of monocyte uPAR expression. Although tumor necrosis factor alpha (TNF) upregulated monocyte uPAR expression, anti-TNF did not influence the endotoxin-induced increase in monocyte uPAR expression. These data suggest that infectious stimuli may influence monocyte function in vivo by enhancing the expression of uPAR.

引用

页码：2156 / 2160

页数：5

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