Development and linkage mapping of E-STS and RGA markers for functional gene homologues in apple

被引：31

作者：

Naik, Suresh

Hampson, Cheryl

Gasic, Ksenija

Bakkeren, Guus

Korban, Schuyler S.

机构：

[1] Agr & Agri Food Canada, Pacific Agri Food Res Ctr, Summerland, BC V0H 1Z0, Canada

[2] Univ Illinois, Dept Nat Resources & Environm Sci, Urbana, IL 61801 USA

来源：

GENOME | 2006年 / 49卷 / 08期

关键词：

apple scab; EST; Malus; RAPD; SSR;

D O I：

10.1139/G06-085

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Linkage maps developed from known-function genes can be valuable in the candidate gene mapping approach. A set of 121 expressed sequence tagged site (E-STS) primer pairs were tested on a framework genetic linkage map of apple (Malus x domestica Borkh.) constructed using simple sequence repeats (SSRs) and randomly amplified polymorphic DNA (RAPD) markers. These known-function gene markers, E-STSs, were supplemented by markers for resistance gene analogues (RGAs), designed based on conserved motifs in all characterized resistance genes isolated from plant species. A total of 229 markers, including 46 apple E-STSs, 8 RGAs, 85 SSRs from apple and peach, and 88 RAPDs, were assigned to 17 linkage groups covering 832 cM of the apple genome, based on 52 individuals originating from the cross 'Antonovka debnicka' (Q12-4) x 'Summerred'. Clusters of E-STS and RGA loci were located in linkage groups previously identified to carry resistance genes, some of which confer resistance to apple scab disease caused by Venturia inaequalis (Cke.) Wint.

引用

页码：959 / 968

页数：10

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