A quantitative method for measuring gene expression magnitude and volume delivered by gene therapy vectors

被引:18
作者
Barton, KN
Xia, XQ
Yan, H
Stricker, H
Heisey, G
Yin, FF
Nagaraja, TN
Zhu, GP
Kolozsvary, A
Fenstermacher, JD
Lu, M
Kim, JH
Freytag, SO
Brown, SL
机构
[1] Henry Ford Hlth System, Dept Radiat Oncol, Detroit, MI 48202 USA
[2] Henry Ford Hlth System, Vattakuti Inst, Detroit, MI 48202 USA
[3] Henry Ford Hlth System, Dept Bioresources, Detroit, MI 48202 USA
[4] Henry Ford Hlth System, Dept Anesthesiol, Detroit, MI 48202 USA
[5] Henry Ford Hlth System, Dept Biostat, Detroit, MI 48202 USA
关键词
adenovirus; nuclear medicine; reporter gene; sodium iodide symporter;
D O I
10.1016/j.ymthe.2004.01.011
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
This study describes a quantitative method to measure the magnitude and distribution of gene expression following local delivery of an adenoviral vector containing the human sodium iodide symporter (hNIS) reporter gene into the canine prostate. Following systemic administration of (NaTcO4)-Tc-99m, autoradiographs of prostate sections depicting hNIS-dependent (TcO4-)-Tc-99m uptake were digitized and stacked to produce a three-dimensional reconstruction of gene expression. Frequency histograms reflecting hNIS gene expression magnitude and volume were used to quantify hNIS function. The method demonstrated submillimeter resolution allowing for precise measurements of gene expression magnitude and volume in vivo. The method developed here could be applied to other reporter gene systems in which the readout can be digitized from thin tissue sections.
引用
收藏
页码:625 / 631
页数:7
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