Mre11 is essential for the maintenance of chromosomal DNA in vertebrate cells

被引：230

作者：

Yamaguchi-Iwai, Y

Sonoda, E

Sasaki, MS

Morrison, C

Haraguchi, T

Hiraoka, Y

Yamashita, YM

Yagi, T

Takata, M

Price, C

Kakazu, N

Takeda, S

机构：

[1] Kyoto Univ, Bayer Chair Dept Mol Immunol & Allergol, Fac Med, Sakyo Ku, Kyoto 6068501, Japan

[2] Kyoto Univ, Ctr Radiat Biol, Sakyo Ku, Kyoto 6068501, Japan

[3] Kansai Adv Res Ctr, Commun Res Lab, Kobe, Hyogo 6512401, Japan

[4] Univ Nebraska, Dept Chem, Lincoln, NE 68588 USA

[5] Univ Nebraska, Dept Biochem, Lincoln, NE 68588 USA

[6] Kyoto Prefectural Univ Med, Kyoto 6020841, Japan

来源：

EMBO JOURNAL | 1999年 / 18卷 / 23期

关键词：

chromosomal aberration; double-strand break repair; DT40; homologous recombination; Mre11;

D O I：

10.1093/emboj/18.23.6619

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Yeast Mre11 functions with Rad50 and Xrs2 in a complex that has pivotal roles in homologous recombination (HR) and non-homologous end-joining (NHEJ) DNA double-strand break (DSB) repair pathways. Vertebrate Mre11 is essential. Conditionally, MRE11 null chicken DT40 cells accumulate chromosome breaks and die upon Mre11 repression, showing frequent centrosome amplification. Mre11 deficiency also causes increased radiosensitivity and strongly reduced targeted integration frequencies. Mre11 is, therefore, crucial for HR and essential in mitosis through its role in chromosome maintenance by recombinational repair Surprisingly perhaps, given the role of Mre11 in yeast NHEJ, disruption of NHEJ by deletion of KU70 greatly exacerbates the effects of MRE11 deficiency, revealing a significant Mre11-independent component of metazoan NHEJ.

引用

页码：6619 / 6629

页数：11

共 65 条

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