Transcriptional analysis of hydrogenase genes in the cyanobacteria Anacystis nidulans and Anabaena variabilis monitored by RT-PCR

被引:50
作者
Boison, G [1 ]
Bothe, H [1 ]
Schmitz, O [1 ]
机构
[1] Univ Cologne, Inst Bot, D-50923 Cologne, Germany
关键词
D O I
10.1007/s002849910063
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Diverse cyanobacteria express an uptake hydrogenase, encoded by the genes hupSL, and a bidirectional, NAD(P)(+)-reducing hydrogenase with the genes hox(E)FUYH. In the unicellular Anacystis nidulans, the hox genes are organized on two separate loci, whereas they are contiguous in one cluster, though interspersed with two unidentified reading frames, ORF 3 and 8, in the heterocystous Anabaena variabilis. The hox gene clusters of these two cyanobacteria have now been transcriptionally analyzed by RT-PCR. A polycistronic transcript was identified in both cyanobacteria. In A. nidulans, one message for each locus has been detected, the dicistronic hoxEF unit, and the polycistronic hoxUYHWhypAB one. In A. variabilis, the transcript consists of the hox genes hoxFUYH as well as the unidentified ORFs. Previous enzyme determinations on the occurrence of the uptake hydrogenase in vegetative cells and thus outside of heterocysts gave ambiguous results. Therefore, transcription of both hup and hox genes has been analyzed in both heterocysts and vegetative cells of A. variabilis. A hupL transcript is detectable in heterocysts and also, though less extensive but clearly discernible, in vegetative cells of NH4+-grown A. variabilis.
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页码:315 / 321
页数:7
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共 41 条
  • [1] APPEL J, 1996, BIOCHIM BIOPHYS ACTA, V1298, P142
  • [2] Transcriptional regulation of Nostoc uptake hydrogenase
    Axelsson, R
    Oxelfelt, F
    Lindblad, P
    [J]. FEMS MICROBIOLOGY LETTERS, 1999, 170 (01) : 77 - 81
  • [3] Unusual gene arrangement of the bidirectional hydrogenase and functional analysis of its diaphorase subunit HoxU in respiration of the unicellular cyanobacterium Anacystis nidulans
    Boison, G
    Schmitz, O
    Schmitz, B
    Bothe, H
    [J]. CURRENT MICROBIOLOGY, 1998, 36 (05) : 253 - 258
  • [4] Evidence against a common use of the diaphorase subunits by the bidirectional hydrogenase and by the respiratory complex I in cyanobacteria
    Boison, G
    Bothe, H
    Hansel, A
    Lindblad, P
    [J]. FEMS MICROBIOLOGY LETTERS, 1999, 174 (01) : 159 - 165
  • [5] Cloning, molecular analysis and insertional mutagenesis of the bidirectional hydrogenase genes from the cyanobacterium Anacystis nidulans
    Boison, G
    Schmitz, O
    Mikheeva, L
    Shestakov, S
    Bothe, H
    [J]. FEBS LETTERS, 1996, 394 (02) : 153 - 158
  • [6] PROGRAMMED DNA REARRANGEMENT OF A CYANOBACTERIAL HUPL GENE IN HETEROCYSTS
    CARRASCO, CD
    BUETTNER, JA
    GOLDEN, JW
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (03) : 791 - 795
  • [7] Rhodobacter capsulatus HypF is involved in regulation of hydrogenase synthesis through the HupUV proteins
    Colbeau, A
    Elsen, S
    Tomiyama, M
    Zorin, NA
    Dimon, B
    Vignais, PM
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1998, 251 (1-2): : 65 - 71
  • [8] ORGANIZATION OF THE GENES NECESSARY FOR HYDROGENASE EXPRESSION IN RHODOBACTER-CAPSULATUS - SEQUENCE-ANALYSIS AND IDENTIFICATION OF 2 HYP REGULATORY MUTANTS
    COLBEAU, A
    RICHAUD, P
    TOUSSAINT, B
    CABALLERO, FJ
    ELSTER, C
    DELPHIN, C
    SMITH, RL
    CHABERT, J
    VIGNAIS, PM
    [J]. MOLECULAR MICROBIOLOGY, 1993, 8 (01) : 15 - 29
  • [9] EISBRENNER G, 1981, J GEN MICROBIOL, V125, P383
  • [10] MODES OF ELECTRON-TRANSFER FROM MOLECULAR-HYDROGEN IN ANABAENA-CYLINDRICA
    EISBRENNER, G
    BOTHE, H
    [J]. ARCHIVES OF MICROBIOLOGY, 1979, 123 (01) : 37 - 45