Assessment of novel problotic Lactobacillus casei strains for the production of functional dairy foods

被引:113
作者
Minelli, EB
Benini, A
Marzotto, M
Sbarbati, A
Ruzzenente, O
Ferrario, R
Hendriks, H
Dellaglio, F
机构
[1] Univ Verona, Dept Med & Publ Hlth, Pharmacol Sect, I-37134 Verona, Italy
[2] Univ Verona, Dept Sci & Technol, I-37134 Verona, Italy
[3] Univ Verona, Dept Morphol & Biomed Sci, I-37134 Verona, Italy
[4] Azienda Osped Verona, I-37134 Verona, Italy
[5] Fattoria Scaldasole, Div PLADA NORD, Monguzzo, Como, Italy
[6] Univ Utrecht, Fac Med Vet, Dept Pathol, Utrecht, Netherlands
关键词
lactobacillus casei; probiotic; fermented milk; hypocholesterolemic; intestinal microflora;
D O I
10.1016/j.idairyj.2004.01.007
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Selected Lactobacillus casei strains were examined for their potential use in new probiotic fermented milks by evaluation of their technological performances, in vitro adhesion capacity and intestinal transit tolerance after administration to rats. Serum, tissue and faecal samples were analysed before and during treatments. The number of intestinal lactobacilli increased significantly after administration of all four strains as verified by standard microbiological analysis, and specific polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). Lactobacillus casei strains were also detected by PCR in faecal samples and specimens of the intestinal epithelium after treatment. Electron microscopy of gut mucosa revealed a thickening of the glycocalyx covering the enterocytes. In all treated rats, levels of serum triglycerides, cholesterol, transaminase and total bilirubin decreased. Microbiological and haematological parameters indicated that all four L. casei strains present favourable strain-specific properties for their utilisation in functional, health-favouring food. (C) 2004 Elsevier Ltd. All rights reserved.
引用
收藏
页码:723 / 736
页数:14
相关论文
共 61 条
  • [1] Probiotic agents and infectious diseases: A modern perspective on a traditional therapy
    Alvarez-Olmos, MI
    Oberhelman, RA
    [J]. CLINICAL INFECTIOUS DISEASES, 2001, 32 (11) : 1567 - 1576
  • [2] Good adhesion properties of probiotics: a potential risk for bacteremia?
    Apostolou, E
    Kirjavainen, PV
    Saxelin, M
    Rautelin, H
    Valtonen, V
    Salminen, SJ
    Ouwehand, AC
    [J]. FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY, 2001, 31 (01): : 35 - 39
  • [3] Bile salt deconjugation and cholesterol removal from media by Lactobacillus casei
    Brashears, MM
    Gilliland, SE
    Buck, LM
    [J]. JOURNAL OF DAIRY SCIENCE, 1998, 81 (08) : 2103 - 2110
  • [4] Camaschella P, 1998, LAIT, V78, P461, DOI 10.1051/lait:1998444
  • [5] Charron M, 1998, INFLAMM BOWEL DIS, V4, P84
  • [6] SURVIVAL OF LACTIC-ACID BACTERIA IN THE HUMAN STOMACH AND ADHESION TO INTESTINAL-CELLS
    CONWAY, PL
    GORBACH, SL
    GOLDIN, BR
    [J]. JOURNAL OF DAIRY SCIENCE, 1987, 70 (01) : 1 - 12
  • [7] Meta-analysis: the effect of probiotic administration on antibiotic-associated diarrhoea
    Cremonini, F
    Di Caro, S
    Nista, EC
    Bartolozzi, F
    Capelli, G
    Gasbarrini, G
    Gasbarrini, A
    [J]. ALIMENTARY PHARMACOLOGY & THERAPEUTICS, 2002, 16 (08) : 1461 - 1467
  • [8] CRITTENDEN R, 2002, MICROBIAL ECOLOGY S, V2, P22
  • [9] DEVELOPMENT OF A DIFFERENTIAL MEDIUM FOR BILE-SALT HYDROLASE-ACTIVE LACTOBACILLUS SPP
    DASHKEVICZ, MP
    FEIGHNER, SD
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1989, 55 (01) : 11 - 16
  • [10] Cholesterol lowering in pigs through enhanced bacterial bile salt hydrolase activity
    De Smet, I
    De Boever, P
    Verstraete, W
    [J]. BRITISH JOURNAL OF NUTRITION, 1998, 79 (02) : 185 - 194