Labeling of stem cells with monocrystalline iron oxide for tracking and localization by magnetic resonance imaging

被引：25

作者：

Calzi, Sergio Li ^{[1
]}

Kent, David L. ^{[2
,3
]}

Chang, Kyung-Hee ^{[1
]}

Padgett, Kyle R. ^{[4
]}

Afzal, Aqeela ^{[1
]}

Chandra, Saurav B. ^{[4
]}

Caballero, Sergio ^{[1
]}

English, Denis ^{[5
]}

Garlington, Wendy ^{[1
]}

Hiscott, Paul S. ^{[2
,3
]}

Sheridan, Carl M. ^{[2
,3
]}

Grant, Maria B. ^{[1
]}

Forder, John R. ^{[4
,6
,7
]}

机构：

[1] Univ Florida, Dept Pharmacol & Therapeut, Program Stem Cell Biol, Gainesville, FL 32610 USA

[2] Univ Liverpool, Dept Med, Unit Ophthalmol, Liverpool L69 3BX, Merseyside, England

[3] Aut Even Hosp, Eye Serv, Kilkenny, Ireland

[4] Univ Florida, McKnight Brain Inst, Gainesville, FL 32610 USA

[5] Univ S Florida, Coll Med, Dept Neurosurg, Tampa, FL 33620 USA

[6] Univ Florida, Dept Radiol, Gainesville, FL 32610 USA

[7] Univ Florida, Dept Biomed Engn, Gainesville, FL 32610 USA

来源：

MICROVASCULAR RESEARCH | 2009年 / 78卷 / 01期

基金：

美国国家卫生研究院;

关键词：

Stem cells; Cell localization; Cell therapy; In vivo tracking; Magnetic resonance imaging; Choroidal neovascularization; MION; EXPERIMENTAL CHOROIDAL NEOVASCULARIZATION; IN-VIVO DETECTION; PROGENITOR CELLS; CONTRAST AGENTS; ANIMAL-MODELS; SINGLE CELLS; MRI; VISUALIZATION; NANOPARTICLES; MIGRATION;

D O I：

10.1016/j.mvr.2009.03.007

中图分类号：

R6 [外科学];

学科分类号：

100210 [外科学];

摘要：

Precise localization of exogenously delivered stem cells is critical to our understanding of their reparative response. Our current inability to determine the exact location of small numbers of cells may hinder optimal development of these cells for clinical use. We describe a method using magnetic resonance imaging to track and localize small numbers of stem cells following transplantation. Endothelial progenitor cells (EPC) were labeled with monocrystalline iron oxide nanoparticles (MIONs) which neither adversely altered their viability nor their ability to migrate in vitro and allowed successful detection of limited numbers of these cells in muscle. MION-labeled stem cells were also injected into the vitreous cavity of mice undergoing the model of choroidal neovascularization, laser rupture of Bruch's membrane. Migration of the MION-labeled cells from the injection site towards the laser burns was visualized by MRI. In conclusion, MION labeling of EPC provides a non-invasive means to define the location of small numbers of these cells. Localization of these cells following injection is critical to their optimization for therapy. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：132 / 139

页数：8

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