A rapid and quantitative method for total fatty acid analysis of fungi and other biological samples

被引:24
作者
Sönnichsen, M [1 ]
Müller, BW [1 ]
机构
[1] Univ Kiel, Dept Pharmaceut & Biopharmaceut, D-24118 Kiel, Germany
关键词
D O I
10.1007/s11745-999-0488-z
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A quantitative method for the one-step esterification and determination of absolute amounts of polyunsaturated fatty acids in biotechnologically produced fungal mycelia is proposed. A system of two internal standards was used to quantify the total fatty acid content of the samples by gas chromatography. The degree of methylation of the analyzed fatty acids was calculated using the internal standards, and subsequently this quotient was used to validate the derivatization and extraction reactions. By utilizing this degree of methylation, the corrected amounts of the various fatty acids in the sample can be calculated.
引用
收藏
页码:1347 / 1349
页数:3
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