Location of 2′-O-methyl nucleotides in 26S rRNA and methylation guide snoRNAs in Caenorhabditis elegans

被引：16

作者：

Higa, S ^{[1
]}

Maeda, N ^{[1
]}

Kenmochi, N ^{[1
]}

Tanaka, T ^{[1
]}

机构：

[1] Univ Ryukyus, Sch Med, Dept Biochem, Okinawa 9030215, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2002年 / 297卷 / 05期

关键词：

rRNA; snoRNA; 2 '-O-methylation; modification; Caenorhabditis elegans;

D O I：

10.1016/S0006-291X(02)02377-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Many nucleotides in rRNAs are modified. We devised a method to locate 2'-O-methyl nucleotide residues using a conventional DNA sequencer. We found 38 2'-O-methyl nucleotides in the 26S rRNA of Caenorhabditis elegans using this method. Fourteen of the 38 residues are conserved in both human and yeast rRNAs and 14 residues are conserved in either human or yeast rRNA. The remaining 10 nucleotides are uniquely methylated in C elegans 26S rRNA. We searched the C elegans genomic sequence for small nucleolar RNAs (snoRNAs), which guide the methylation of ribose residues, and predicted 18 snoRNA sequences that are expected to guide the methylation of some of these nucleotide residues. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：1344 / 1349

页数：6

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