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Optimization of the production of an extracellular alkaline protease from Bacillus horikoshii

被引：167
作者：
Joo, HS
Kumar, CG
Park, GC
Kim, KT
Paik, SR
Chang, CS
机构：
[1] Inha Univ, Coll Med, Dept Biochem, Chung Ku, Inchon 400103, South Korea
[2] Inha Univ, Inst Med Sci, Chung Ku, Inchon 400103, South Korea
[3] Seoul Biosci Inst, Gangdong Gu, Seoul 134030, South Korea
来源：
PROCESS BIOCHEMISTRY | 2002年 / 38卷 / 02期
关键词：
Bacillus horikoshii; Polychaeta; alkaline protease; soybean meal;
D O I：
10.1016/S0032-9592(02)00061-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 [生物化学与分子生物学]; 081704 [应用化学];
摘要：
Bacillus horikoshii which produces an extracellular alkaline protease, was isolated from the body fluid of an unique Korean polychaeta (Periserrula leucophryna) living in the tidal mud flats of Kwangwha island in the Korean West Sea (Yellow Sea). Maximum enzyme activity was achieved when the bacterium was grown in soybean meal (1.5%, w/v) and casein (1%, w/v) at pH 9.0 and 34 degreesC over 16-18 h incubation period. The enzyme has an optimum pH of around 9 and maintained its stability over a broad pH range between 5.5 and 12. Its optimum temperature is around 45 degreesC, and exhibited a stability of up to 50 degreesC. The enzyme activity was strongly inhibited by PMSF, suggesting that it belongs to the family of serine proteases. (C) 2002 Elsevier Science Ltd. All rights reserved.
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页码：155 / 159
页数：5
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