The role of phosphatidylinositide-3-kinase in basal mitogen-activated protein kinase activity and cell survival

被引：22

作者：

Versteeg, HH ^{[1
]}

Evertzen, MWA ^{[1
]}

van Deventer, SJH ^{[1
]}

Peppelenbosch, MP ^{[1
]}

机构：

[1] Univ Amsterdam, Acad Med Ctr, Lab Expt Internal Med, NL-1105 AZ Amsterdam, Netherlands

来源：

FEBS LETTERS | 2000年 / 465卷 / 01期

关键词：

PI; 3-kinase; constitutive MAPK activation; wortmannin; LY294002; cell survival;

D O I：

10.1016/S0014-5793(99)01701-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phosphatidylinositide-3-OH-kinase (PI 3-kinase) is an upstream activator of p42/p44 mitogen-activated protein kinase (MAPK), but the role of PI 3-kinase-dependent MAPK remains obscure. Here we demonstrate that in a variety of different cell types, PI 3-kinase inhibition results in an inhibition of MAPK in unstimulated cells but does not interfere with growth factor, or TPA-induced MAPK activity. Furthermore, inhibition of either PI 3-kinase or MEK/MAPK results in cell death in serum-starved cells, We concluded that basal, but not induced MAPK activity is mediated by PI 3-kinase and that this PI 3-kinase-mediated MEK/MAPK activity is essential for cell survival in quiescent cells. (C) 2000 Federation of European Biochemical Societies.

引用

页码：69 / 73

页数：5