A sensitive endocannabinoid assay.: The simultaneous analysis of N-acylethanolamines and 2-monoacylglycerols

被引：68

作者：

Schmid, PC ^{[1
]}

Schwartz, KD ^{[1
]}

Smith, CN ^{[1
]}

Krebsbach, RJ ^{[1
]}

Berdyshev, EV ^{[1
]}

Schmid, HHO ^{[1
]}

机构：

[1] Univ Minnesota, Hormel Inst, Austin, MN 55912 USA

来源：

CHEMISTRY AND PHYSICS OF LIPIDS | 2000年 / 104卷 / 02期

关键词：

N-acylethanolamine; anandamide; 2-monoacylglycerol; 2-arachidonoylglycerol; mass spectrometry; rat organs; JB6; P+ cells;

D O I：

10.1016/S0009-3084(99)00124-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mammalian cells produce both N-arachidonoylethanolamine (20:4n-6 NAE, anandamide) and 2-arachidonoylglycerol (2-AG), lipid signaling molecules that activate cannabinoid receptors. Because both agonists occur in the presence of receptor-inactive congeners, we have developed a sensitive method for the simultaneous assay of N-acylethanolamines (NAEs) and 2-monoacylglycerols (2-MAG). These lipid classes are isolated from total lipids by solid phase extraction and converted to tert-butyldimethylsilyl (tBDMS) derivatives in the presence of deuterated analogs. The tBDMS derivatives are analyzed by gas chromatography/mass spectrometry using selected ion monitoring programs specific for NAE and 2-MAG. Individual NAEs and 2-MAGs can be quantified in the nanogram and subnanogram range. The NAE and 2-MAG compositions of rat organs and cultured JB6 cells are reported. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

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页码：185 / 191

页数：7

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