Application of loop-mediated isothermal amplification for detection of Yersinia enterocolitica in pork meat

被引：37

作者：

Gao, Hongwei ^{[1
]}

Lei, Zhiwen ^{[1
]}

Jia, Juntao ^{[1
]}

Wang, Shufeng ^{[1
]}

Chen, Ying ^{[2
]}

Sun, Min ^{[1
]}

Liang, Chengzhu ^{[1
]}

机构：

[1] Shandong Entry Exit Inspect & Quarantine Bur Peop, Qingdao, Peoples R China

[2] Chinese Acad Inspect & Quarantine, Inst Food Safety, Beijing, Peoples R China

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2009年 / 77卷 / 02期

关键词：

Loop-mediated isothermal amplification; Detection; Yersinia enterocolitica; PIG TONSILS; RAPID DETECTION; PCR; FOOD; PSEUDOTUBERCULOSIS; IDENTIFICATION; CULTURE; SAMPLES; FIELD; SPP;

D O I：

10.1016/j.mimet.2009.02.001

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A loop-mediated isothermal amplification (LAMP) method was developed for the detection of Yersinia enterocolitica isolates in both pure bacterial cultures and pork meat. The LAMP primers, which corresponded to the gyrB gene, accurately identified 4 different bioserotypes of Y. enterocolitica. These primers failed to detect Y. pseudotuberculosis, Y. frederiksenii, and 17 non-Yersinia strains. The sensitivity of the LAMP assay for the detection of Y. enterocolitica in pure culture was 65 CFU/mL (31.6 fg of genomic DNA). The LAMP assay was conducted for the detection of Y. enterocolitica strains in 21 pig tonsil samples and 73 pork meat samples obtained from 94 slaughtered pigs belonging to 4 different herds. Y. enterocolitica was found to be present in 4 tonsil samples and none in meat samples. This is the first report in which the LAMP assay was employed for the detection of Y. enterocolitica in food samples. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：198 / 201

页数：4

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