Trans-7,cis-9 CLA is synthesized endogenously by △9-desaturase in dairy cows

被引:92
作者
Corl, BA
Baumgard, LH
Griinari, JM
Delmonte, P
Morehouse, KM
Yuraweczc, MP
Bauman, DE
机构
[1] Cornell Univ, Dept Anim Sci, Ithaca, NY 14853 USA
[2] Univ Helsinki, Dept Anim Sci, FIN-00014 Helsinki, Finland
[3] US FDA, Ctr Food Safety & Appl Nutr, Washington, DC 20204 USA
关键词
D O I
10.1007/s11745-002-0949-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cis-9,trans-11 and traps-7,cis-9 CLA are the most prevalent CLA isomers in milkfat. The majority of cis-9,trans-11 CLA is synthesized endogenously by Delta(9)-desaturase. We tested the hypothesis that traps-7,cis-9 CLA originates from endogenous synthesis by inhibiting Delta(9)-desaturase with a source of cyclopropene FA (sterculic oil: SO) or with a trans-10,cis-12 CLA supplement. Experiment 1 (four cows; Latin square) involved four treatments: control, SO, partially hydrogenated vegetable oil (PHVO), and PHVO + SO. Milk, plasma, and rumen fluid were collected. Experiment 2 treatments (four cows) were 0 or 14.0 g/d of 10,12 CLA supplement; milk and plasma were collected. Samples were analyzed by GC and Ag+-HPLC to determine FA. In Experiment 1, SO decreased milkfat content of traps-7,cis-9 CLA by 68 to 71% and cis-9,trans-11 CLA by 61 to 65%. In Experiment 2, the 10,12 CLA supplement decreased milkfat content of traps-7,cis-9 CLA and cis-9,trans-11 by 44 and 25%, respectively. Correcting for the extent of treatment-induced inhibition of Delta(9)-desaturase based on changes in myristic and myristoleic acids, endogenous synthesis of traps-7,cis-9 CLA represented 85 and 102% in Experiments 1 and 2, respectively. Similar corrected values were 77 and 58% for endogenous synthesis of cis-9,trans-11 CLA. Thus, milkfat cis-9,trans-11 CLA was primarily from endogenous synthesis with a minor portion from rumen escape. In contrast, traps-7,cis-9 CLA was not present in rumen fluid in significant amounts. Results indicate this isomer in milkfat is derived almost exclusively from endogenous synthesis via Delta(9)-desaturase.
引用
收藏
页码:681 / 688
页数:8
相关论文
共 49 条
[1]  
[Anonymous], 1989, SAS STAT US GUID VER
[2]  
[Anonymous], 1989, Nutrient requirements of dairy cattle
[3]   Method for extraction and separation by solid phase extraction of neutral lipid, free fatty acids, and polar lipid from mixed microbial cultures [J].
Bateman, HG ;
Jenkins, TC .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1997, 45 (01) :132-134
[4]  
Bauman D., 2013, Lactation: a comprehensive treatise, V2, P31
[5]  
Bauman DE, 2001, LIVEST PROD SCI, V70, P15, DOI 10.1016/S0301-6226(01)00195-6
[6]   Identification of the conjugated linoleic acid isomer that inhibits milk fat synthesis [J].
Baumgard, LH ;
Corl, BA ;
Dwyer, DA ;
Saebo, A ;
Bauman, DE .
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, 2000, 278 (01) :R179-R184
[7]   Milk fat synthesis in dairy cows is progressively reduced by increasing supplemental amounts of trans-10, cis-12 conjugated linoleic acid (CLA) [J].
Baumgard, LH ;
Sangster, JK ;
Bauman, DE .
JOURNAL OF NUTRITION, 2001, 131 (06) :1764-1769
[8]  
BAUMGARD LH, 2002, IN PRESS J DAIRY SCI, P85
[9]   GLYCEROKINASE AND DESATURASE ACTIVITY IN PIG, CHICKEN AND SHEEP INTESTINAL EPITHELIUM [J].
BICKERSTAFFE, R ;
ANNISON, EF .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY, 1969, 31 (01) :47-+
[10]   EFFECT OF INTRAVENOUS INFUSIONS OF STERCULIC ACID ON MILK-FAT SYNTHESIS [J].
BICKERSTAFFE, R ;
JOHNSON, AR .
BRITISH JOURNAL OF NUTRITION, 1972, 27 (03) :561-+