Selectively infective phage (SIP) technology:: scope and limitations

被引：23

作者：

Jung, S ^{[1
]}

Arndt, KM ^{[1
]}

Müller, KM ^{[1
]}

Plückthun, A ^{[1
]}

机构：

[1] Univ Zurich, Inst Biochem, CH-8057 Zurich, Switzerland

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1999年 / 231卷 / 1-2期

关键词：

phage display; affinity maturation; disulfide bonds; ScFv fragments;

D O I：

10.1016/S0022-1759(99)00143-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We review here the selectively infective phage (SIP) technology, a powerful tool for the rapid selection of protein-ligand and peptide-ligand pairs with very high affinities. SIP is highly suitable for discriminating between molecules with subtle stability and folding differences. We discuss the preferred types of applications for this technology and some pitfalls inherent in the in vivo SIP method that have become apparent in its application with highly randomized libraries, as well as some precautions that should be taken in successfully applying this technology. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

收藏

页码：93 / 104

页数：12

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