CRYSTAL-STRUCTURE OF A CHIMERIC FAB' FRAGMENT OF AN ANTIBODY-BINDING TUMOR-CELLS

被引：50

作者：

BRADY, RL ^{[1
]}

EDWARDS, DJ ^{[1
]}

HUBBARD, RE ^{[1
]}

JIANG, JS ^{[1
]}

LANGE, G ^{[1
]}

ROBERTS, SM ^{[1
]}

TODD, RJ ^{[1
]}

ADAIR, JR ^{[1
]}

EMTAGE, JS ^{[1
]}

KING, DJ ^{[1
]}

LOW, DC ^{[1
]}

机构：

[1] CELLTECH LTD,DIV CELLTECH RES,SLOUGH SL1 4EN,ENGLAND

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1992年 / 227卷 / 01期

关键词：

ANTIBODY; FAB'FRAGMENT; PROTEIN CRYSTALLOGRAPHY; MOLECULAR REPLACEMENT; ANTIBODY HUMANIZATION;

D O I：

10.1016/0022-2836(92)90695-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The crystal structure of a chimeric Fab′ fragment of a monoclonal antibody is presented. The Fab′ comprises the murine light chain and heavy chain variable domains of the carcinoma-binding antibody B72.3 fused to the constant domain of human κ, and the first constant domain and hinge domain of human γ4, respectively. A model for the Fab′ has been determined by molecular replacement and refined to a resolution of 3·1 Å with an R-factor of 17·6%. The additional residues that distinguish a Fab′ from a Fab fragment are seen to be disordered in the crystals. The H3 hypervariable loop is short and adopts a sharp hairpin turn in a conformation that results from an interaction between the lysine side-chain of H93 and the main-chain carbonyl group of H96. The remaining hypervariable loops display conformations similar to those predicted from the canonical structures approach, although loop H2 is apparently displaced by a salt-bridge formed between H55 Asp and the neighbouring H73 Lys. These and other features of the structure likely to be important in grafting the hypervariable loops to an otherwise human framework are discussed. © 1992.

引用

页码：253 / 264

页数：12

共 43 条

[1]

BAKER TS, 1992, IN PRESS ANTIBODY IM

[2]

BEGENT RHJ, 1990, BRIT J CANCER, V62, P487

[3] CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDY OF A CHIMERIC FAB' FRAGMENT OF ANTIBODY-BINDING TUMOR-CELLS [J].