CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDY OF A CHIMERIC FAB' FRAGMENT OF ANTIBODY-BINDING TUMOR-CELLS

被引：22

作者：

BRADY, RL ^{[1
]}

HUBBARD, RE ^{[1
]}

KING, DJ ^{[1
]}

LOW, DC ^{[1
]}

ROBERTS, SM ^{[1
]}

TODD, RJ ^{[1
]}

机构：

[1] CELLTECH LTD,SLOUGH SL1 4EN,ENGLAND

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1991年 / 219卷 / 04期

关键词：

CRYSTALLIZATION; MONOCLONAL ANTIBODY; FAB' FRAGMENT; CHIMERIC ANTIBODY; TUMOR CELLS;

D O I：

10.1016/0022-2836(91)90656-Q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Crystals have been obtained of a chimaeric Fabt′ fragment that binds to a tumour-associated mucin-like glycoprotein TAG72. The Fab′ fragment comprises the variable heavy and light-chain domains of a murine monoclonal antibody, B72.3, coupled to human γ4 and κ constant regions. The crystals are orthorhombic and belong to the space group P212121, with unit cell dimensions a = 67.9 A ̊, b = 94.2 A ̊ and c = 208.8 A ̊. Diffraction to 2.6 Å resolution was observed using synchrotron radiation. Despite the acute radiation sensitivity of the crystals a full native data set has been collected using the Weissenberg camera at the Photon Factory synchrotron. These data will be used for molecular replacement calculations in an attempt to elucidate the structure of this chimaeric Fab′ fragment. © 1991.

引用

页码：603 / 604

页数：2

共 10 条

[1]

BROWN BA, 1987, CANCER RES, V47, P1149

[2] A SPECTRUM OF MONOCLONAL-ANTIBODIES REACTIVE WITH HUMAN MAMMARY-TUMOR CELLS [J].