FluidFM: Combining Atomic Force Microscopy and Nanofluidics in a Universal Liquid Delivery System for Single Cell Applications and Beyond

被引：332

作者：

Meister, Andre ^{[2
]}

Gabi, Michael ^{[1
]}

Behr, Pascal ^{[1
]}

Studer, Philipp ^{[1
]}

Voeroes, Janos ^{[1
]}

Niedermann, Philippe ^{[2
]}

Bitterli, Joanna ^{[2
]}

Polesel-Maris, Jerome ^{[2
]}

Liley, Martha ^{[2
]}

Heinzelmann, Harry ^{[2
]}

Zambelli, Tomaso ^{[1
]}

机构：

[1] ETH, Inst Biomed Engn, Lab Biosensors & Bioelect, CH-8092 Zurich, Switzerland

[2] CSEM SA, Swiss Ctr Elect & Microtechnol, CH-2002 Neuchatel, Switzerland

来源：

NANO LETTERS | 2009年 / 9卷 / 06期

关键词：

MICROMACHINED FOUNTAIN PEN; RESOLUTION; SURFACE;

D O I：

10.1021/nl901384x

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We describe the fluidFM, an atomic force microscope (AFM) based on hollow cantilevers for local liquid dispensing and stimulation of single living cells under physiological conditions. A nanofluldic channel in the cantilever allows soluble molecules to be dispensed through a submicrometer aperture in the AFM tip. The sensitive AFM force feedback allows controlled approach of the tip to a sample for extremely local modification of surfaces in liquid environments. It also allows reliable discrimination between gentle contact with a cell membrane or its perforation. Using these two procedures, dyes have been introduced into individual living cells and even selected subcellular structures of these cells. The universality and versatility of the fluidFM will stimulate original experiments at the submicrometer scale not only in biology but also in physics, chemistry, and material science.

引用

页码：2501 / 2507

页数：7

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