High-performance liquid chromatographic enantio separations on monolithic silica columns containing a covalently attached 3,5-dimethylphenylcarbamate derivative of cellulose

被引：103

作者：

Chankvetadze, B

Ikai, T

Yamamoto, C

Okamoto, Y

机构：

[1] Tbilisi State Univ, Sch Chem, Mol Recognit & Separat Sci Lab, GE-380028 Tbilisi, Georgia

[2] Nagoya Univ, Grad Sch Engn, Dept Appl Chem, Nagoya, Aichi 4648603, Japan

来源：

JOURNAL OF CHROMATOGRAPHY A | 2004年 / 1042卷 / 1-2期

关键词：

enantiomer separation; monolithic columns; cellulose dimethylphenylcarbamate; trifluoroanthrylethanol; cobalt tris(acetylacetonate); dihydroxy dimethylbiphenyl;

D O I：

10.1016/j.chroma.2004.05.011

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Covalent immobilization of 3,5-dimethylphenylcarbamate derivative of cellulose was performed in situ onto native silica monoliths cladded in a 50 mm x 4.6 mm polyether ether ketone high-performance liquid chromatographic (HPLC) column. The covalent attachment of cellulose derivative in the range of 16-19% (w/w) was performed via an epoxide moiety. The column obtained by this technique combines the high enantiomer-resolving ability of the polysaccharide derivative with favourable dynamic properties of monolithic HPLC columns. The covalent attachment of the cellulose derivative enables this column to be used in combination with the mobile phases which are incompatible with coated-type polysaccharide columns due to solubility of chiral selector in some organic solvents. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：55 / 60

页数：6

共 32 条

[1] Comparative study on the column performance of microparticulate 5-μm C18-bonded and monolithic C18-bonded reversed-phase columns in high-performance liquid chromatography [J].