Th2 activities induced during virgin T cell priming in the absence of IL-4, IL-13, and B cells

被引:41
作者
Cunningham, AF
Fallon, PG
Khan, M
Vacheron, S
Acha-Orbea, H
MacLennan, ICM [1 ]
McKenzie, AN
Toellner, KM
机构
[1] Univ Birmingham, MRC, Ctr Immune Regulat, Birmingham B15 2TT, W Midlands, England
[2] Univ Cambridge, Dept Pathol, Cambridge CB2 1QP, England
[3] Univ Lausanne, Ludwig Inst Canc Res, CH-1066 Epalinges, Switzerland
[4] Univ Lausanne, Inst Biochem, CH-1066 Epalinges, Switzerland
[5] MRC, Mol Biol Lab, Cambridge, England
关键词
D O I
10.4049/jimmunol.169.6.2900
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Virgin T cells being primed to Th2-inducing or Th1-inducing Ags, respectively, start to synthesize IL-4 or IFN-gamma as they begin to proliferate. Parallel respective induction of B cells to produce gamma1 or gamma2a switch transcripts provides additional evidence of early divergent Th activity. This report concerns the roles of IL-4, IL-13, and B cells in these early events in vivo. Th2 responses were induced in lymph nodes against hapten-protein given s.c. with killed Bordetella pertussis adjuvant. In T cell proliferation in wild-type mice, IL-4 message up-regulation and gamma1 and epsilon switch transcript production were underway 48-72 111 after immunization. The absence of IL-4, IL-13, or B cells did not alter the early T cell proliferative response. The gamma1 and epsilon switch transcript production was still induced in the absence of IL-4, IL-13, or both, but at a reduced level, while the dominance of switching to IgG1 in the extrafollicular hapten-specific plasma cell response was retained. The up-regulation of IL-4 message was not reduced or delayed in the absence of B cells and was only marginally reduced by the absence of IL-13. It is concluded that signals delivered by dendritic cells, which are not dependent on the presence of IL-4, IL-13, or B cells, can prime virgin T cells and induce the early Th2 activities studied. These early events that direct virgin T cells toward Th2 differentiation contrast with the critical later role of Th2 cytokines in selectively expanding Th2 clones and driving further IL-4 synthesis.
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页码:2900 / 2906
页数:7
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