Label-free assays on the BIND system

被引：195

作者：

Cunningham, BT ^{[1
]}

Li, P ^{[1
]}

Schulz, S ^{[1
]}

Lin, B ^{[1
]}

Baird, C ^{[1
]}

Gerstenmaier, J ^{[1
]}

Genick, C ^{[1
]}

Wang, F ^{[1
]}

Fine, E ^{[1
]}

Laing, L ^{[1
]}

机构：

[1] SRU Biosyst, Woburn, MA 01801 USA

来源：

JOURNAL OF BIOMOLECULAR SCREENING | 2004年 / 9卷 / 06期

关键词：

Biomolecular Interaction Detection system; BIND; biosensors; pharmaceutical screening; label-free detection;

D O I：

10.1177/1087057104267604

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Screening of biochemical interactions becomes simpler, less expensive, and more accurate when labels, such as fluorescent dyes, radioactive markers, and colorimetric reactions, are not required to quantify detected material. SRU Biosystems has developed a biosensor technology that is manufactured on continuous sheets of plastic film and incorporated into standard microplates and microarray slides to enable label-free assays to be performed with high throughput, high sensitivity, and low cost per assay. The biosensor incorporates a narrowband guided-mode resonance reflectance filter, in which the reflected color is modulated by the attachment/detachment of biochemical material to the surface. The technology offers 4 orders of linear dynamic range and uniformity within a plate, with a coefficient of variation of 2.5%. Using conventional biochemical immobilization surface chemistries, a wide range of assay applications are enabled. Small molecule screening, cell proliferation/cytotoxicity, enzyme activity screening, protein-protein interaction, and cell membrane receptor expression are among the applications demonstrated.

引用

页码：481 / 490

页数：10

共 26 条

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