Genomic Characterization of Human Rotavirus G8 Strains From the African Rotavirus Network: Relationship to Animal Rotaviruses

被引:74
作者
Esona, M. D. [1 ]
Geyer, A. [10 ]
Page, N. [2 ]
Trabelsi, A. [3 ]
Fodha, I. [3 ]
Aminu, M. [4 ]
Agbaya, V. A. [5 ]
Tsion, B. [6 ]
Kerin, T. K. [1 ]
Armah, G. E. [7 ]
Steele, A. D. [8 ]
Glass, R. I. [9 ]
Gentsch, J. R. [1 ]
机构
[1] CDC, NCIRD, Div Viral Dis, Gastroenteritis & Resp Viruses Lab Branch, Atlanta, GA 30333 USA
[2] Natl Inst Communicable Dis, Viral Gastroenteritis Unit, Johannesburg, South Africa
[3] Univ Hosp Sahloul, Lab Bacteriol Virol, Sousse, Tunisia
[4] Ahmadu Bello Univ, Dept Microbiol, Zaria, Nigeria
[5] Inst Pasteur Cote dIvoire DVE, Lab Bacteriol Virol, Abidjan, Cote Ivoire
[6] Ethiopian Inst Virol, Addis Ababa, Ethiopia
[7] Noguchi Mem Res Inst, Accra, Ghana
[8] NW, PATH, Seattle, WA USA
[9] NIH, Fogarty Int Ctr, Bethesda, MD 20892 USA
[10] Univ Limpopo, MRC, DPRU, Limpopo, South Africa
关键词
rotavirus; characterization; classification; evolution; reassortment; GROUP-A ROTAVIRUS; RNA-RNA HYBRIDIZATION; AMINO-ACID-SEQUENCE; POLYMERASE CHAIN-REACTION; PORCINE ROTAVIRUS; MONOCLONAL-ANTIBODIES; NONSTRUCTURAL PROTEIN; UNITED-KINGDOM; MOLECULAR CHARACTERIZATION; INTERSPECIES TRANSMISSION;
D O I
10.1002/jmv.21468
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Global rotavirus surveillance has led to the detection of many unusual human rotavirus (HRV) genotypes. During 1996-2004 surveillance within the African Rotavirus Network (ARN), six P[8],G8 and two P[6],G8 human rotavirus strains were identified. Gene fragments (RT-PCR amplicons) of all 11-gene segments of these G8 strains were sequenced in order to elucidate their genetic and evolutionary relationships. Phylogenetic and sequence analyses of each gene segment revealed high similarities (88-100% nt and 91-100% aa) for all segments except for gene 4 encoding VP4 proteins P[8] and P[6]. For most strains, almost all of the genes of the ARN strains other than neutralizing antigens are related to typical human strains of Wa genogroup. The VP7, NSP2, and NSP5 genes were closely related to cognate genes of animal strains (83-99% and 97-99% aa identity). This study suggests that the ARN G8 strains might have arisen through VP7 or VP4 gene reassortment events since most of the other gene segments resemble those of common human rotaviruses. However, VP7, NSP2 (likely), and NSP5 (likely) genes are derived potentially from animals consistent with a zoonotic introduction. Although these findings help elucidate rotavirus evolution, sequence studies of cognate animal rotavirus genes are needed to conclusively determine the specific origin of those genes relative to both human and animal rotavirus strains. J. Med. Virol. 81:937-951, 2009. (C) 2009 Wiley-Liss, Inc.
引用
收藏
页码:937 / 951
页数:15
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