Triacylglycerol hydrolase: role in intracellular lipid metabolism

被引：101

作者：

Dolinsky, VW

Gilham, D

Alam, M

Vance, DE

Lehner, R ^{[1
]}

机构：

[1] Univ Alberta, Heritage Med Res Ctr 328, Dept Pediat, CIHR Grp Mol & Cell Biol Lipids, Edmonton, AB T6G 2S2, Canada

[2] Univ Alberta, Heritage Med Res Ctr 328, Dept Cell Biol, CIHR Grp Mol & Cell Biol Lipids, Edmonton, AB T6G 2S2, Canada

[3] Univ Alberta, Heritage Med Res Ctr 328, Dept Biochem, CIHR Grp Mol & Cell Biol Lipids, Edmonton, AB T6G 2S2, Canada

来源：

CELLULAR AND MOLECULAR LIFE SCIENCES | 2004年 / 61卷 / 13期

关键词：

lipase; carboxylesterase; triacylglycerol; VLDL; liver; adipose; atherosclerosis;

D O I：

10.1007/s00018-004-3426-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent scientific advances have revealed the identity of several enzymes involved in the synthesis, storage and catabolism of intracellular neutral lipid storage droplets. An enzyme that hydrolyzes stored triacylglycerol (TG), triacylglycerol hydrolase (TGH), was purified from porcine, human and murine liver microsomes. In rodents, TGH is highly expressed in liver as well as heart, kidney, small intestine and adipose tissues, while in humans TGH is mainly expressed in the liver, adipose and small intestine. TGH localizes to the endoplasmic reticulum and lipid droplets. The TGH genes are located within a cluster of carboxylesterase genes on human and mouse chromosomes 16 and 8, respectively. TGH hydrolyzes stored TG, and in the liver, the lipolytic products are made available for VLDL-TG synthesis. Inhibition of TGH activity also inhibits TG and apolipoprotein B secretion by primary hepatocytes. A role for TGH in basal TG lipolysis in adipocytes has been proposed. TGH expression and activity is both developmentally and hormonally regulated. A model for the function of TGH is presented and discussed with respect to tissue specific functions.

引用

页码：1633 / 1651

页数：19

共 155 条

[81] CLONING AND EXPRESSION OF HUMAN LECITHIN-CHOLESTEROL ACYLTRANSFERASE CDNA [J].

MCLEAN, J ;

FIELDING, C ;

DRAYNA, D ;

DIEPLINGER, H ;

BAER, B ;

KOHR, W ;

HENZEL, W ;

LAWN, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (08) :2335-2339

[82] Carboxylesterases moonlight in the male reproductive tract: A functional shift pivotal for male fertility [J].

Mikhailov, AT ;

Torrado, M .

FRONTIERS IN BIOSCIENCE-LANDMARK, 2000, 5 :E53-E62

[83] REGULATION OF 2 RAT-LIVER MICROSOMAL CARBOXYLESTERASE ISOZYMES - SPECIES-DIFFERENCES, TISSUE DISTRIBUTION, AND THE EFFECTS OF AGE, SEX, AND XENOBIOTIC TREATMENT OF RATS [J].

MORGAN, EW ;

YAN, BF ;

GREENWAY, D ;

PARKINSON, A .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1994, 315 (02) :513-526

[84] RELATIONSHIP BETWEEN CIRCADIAN-RHYTHM OF FOOD-INTAKE AND THAT OF PLASMA CORTICOSTERONE AND EFFECT OF FOOD RESTRICTION ON CIRCADIAN ADRENOCORTICAL RHYTHM IN RAT [J].

MORIMOTO, Y ;

ARISUE, K ;

YAMAMURA, Y .

NEUROENDOCRINOLOGY, 1977, 23 (04) :212-222

[85] AN ISOZYME OF MICROSOMAL CARBOXYESTERASES, CARBOXYESTERASE SEC, IS SECRETED FROM RAT-LIVER INTO THE BLOOD [J].

MURAKAMI, K ;

TAKAGI, Y ;

MIHARA, K ;

OMURA, T .

JOURNAL OF BIOCHEMISTRY, 1993, 113 (01) :61-66

[86] The biogenesis and functions of lipid bodies in animals, plants and microorganisms [J].

Murphy, DJ .

PROGRESS IN LIPID RESEARCH, 2001, 40 (05) :325-438

[87] Mechanisms of lipid body formation [J].

Murphy, DJ ;

Vance, J .

TRENDS IN BIOCHEMICAL SCIENCES, 1999, 24 (03) :109-115

[88]

Natarajan R, 1999, J LIPID RES, V40, P2091

[89] Molecular cloning of the promoter for rat hepatic neutral cholesterol ester hydrolase: Evidence for transcriptional regulation by sterols [J].

Natarajan, R ;

Ghosh, S ;

Grogan, WM .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1998, 243 (02) :349-355

[90] MOLECULAR-CLONING AND SEQUENCE-ANALYSIS OF CDNA-ENCODING HUMAN CHOLESTEROL 7-ALPHA-HYDROXYLASE [J].

NOSHIRO, M ;

OKUDA, K .

FEBS LETTERS, 1990, 268 (01) :137-140

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