Curcumin inhibits telomerase activity through human telomerase reverse transcritpase in MCF-7 breast cancer cell line

被引：127

作者：

Ramachandran, C

Fonseca, HB

Jhabvala, P

Escalon, EA

Melnick, SJ

机构：

[1] Miami Childrens Hosp, Dept Pathol, Miami, FL 33155 USA

[2] Miami Childrens Hosp, Res Inst, Miami, FL 33155 USA

[3] Miami Childrens Hosp, Div Hematol & Oncol, Miami, FL 33155 USA

来源：

CANCER LETTERS | 2002年 / 184卷 / 01期

关键词：

curcumin; telomerase; human telomerase reverse transcriptase; telomerase RNA; c-myc; real-time reverse transcription-polymerase chain reaction;

D O I：

10.1016/S0304-3835(02)00192-1

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The inhibitory effect of curcumin, the yellow-colored pigment from turmeric, on telomerase activity was analyzed in human mammary epithelial (MCF-10A) and breast cancer (MCF-7) cells. Telomerase activity in MCF-7 cells is 6.9-fold higher than that of human mammary epithelial cells. In MCF-7 cells, telomerase activity decreased with increasing concentrations of curcumin, inhibiting about 93.4% activity at 100 muM concentration. The inhibition of telomerase activity in MCF-7 cells may be due to down-regulation of hTERT expression. Increasing concentrations of curcumin caused a steady decrease in the level of hTERT mRNA in MCF-7 cells whereas the level of hTER and c-myc mRNAs remained the same. Our results suggest that curcumin inhibits telomerase activity by down-regulating hTERT expression in breast cancer cells and this down-regulation is not through the c-myc pathway. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：1 / 6

页数：6

共 41 条

[21] 3 INHIBITORS OF TYPE-1 HUMAN-IMMUNODEFICIENCY-VIRUS LONG TERMINAL REPEAT-DIRECTED GENE-EXPRESSION AND VIRUS-REPLICATION
LI, CJ
ZHANG, LJ
DEZUBE, BJ
CRUMPACKER, CS
PARDEE, AB
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (05) : 1839 - 1842
[22] Lin Z, 2001, AM J PATHOL, V159, P405
[23] INHIBITORY EFFECTS OF CURCUMIN ON PROTEIN-KINASE-C ACTIVITY INDUCED BY 12-O-TETRADECANOYL-PHORBOL-13-ACETATE IN NIH 3T3 CELLS
LIU, JY
LIN, SJ
LIN, JK
[J]. CARCINOGENESIS, 1993, 14 (05) : 857 - 861
[24] EFFECT OF CURCUMIN ON 12-O-TETRADECANOYLPHORBOL-13-ACETATE-INDUCED AND ULTRAVIOLET-B LIGHT-INDUCED EXPRESSION OF C-JUN AND C-FOS IN JB6 CELLS AND IN MOUSE EPIDERMIS
LU, YP
CHANG, RL
LOU, YR
HUANG, MT
NEWMARK, HL
REUHL, KR
CONNEY, AH
[J]. CARCINOGENESIS, 1994, 15 (10) : 2363 - 2370
[25] hEST2, the putative human telomerase catalytic subunit gene, is up-regulated in tumor cells and during immortalization
Meyerson, M
Counter, CM
Eaton, EN
Ellisen, LW
Steiner, P
Caddle, SD
Ziaugra, L
Beijersbergen, RL
Davidoff, MJ
Liu, QY
Bacchetti, S
Haber, DA
Weinberg, RA
[J]. CELL, 1997, 90 (04) : 785 - 795
[26] Telomerase activation by hTRT in human normal fibroblasts and hepatocellular carcinomas
Nakayama, JI
Tahara, H
Tahara, E
Saito, M
Ito, K
Nakamura, H
Nakanishi, T
Tahara, E
Ide, T
Ishikawa, F
[J]. NATURE GENETICS, 1998, 18 (01) : 65 - 68
[27] Inhibition of tissue factor gene activation in cultured endothelial cells by curcumin -: Suppression of activation of transcription factors Egr-1, AP-1, and NF-κB
Pendurthi, UR
Williams, JT
Rao, LVM
[J]. ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 1997, 17 (12) : 3406 - 3413
[28] 1,4- and 2,6-disubstituted amidoanthracene-9,10-dione derivatives as inhibitors of human telomerase
Perry, PJ
Gowan, SM
Reszka, AP
Polucci, P
Jenkins, TC
Kelland, LR
Neidle, S
[J]. JOURNAL OF MEDICINAL CHEMISTRY, 1998, 41 (17) : 3253 - 3260
[29] Human telomerase inhibition by regioisomeric disubstituted amidoanthracene-9,10-diones
Perry, PJ
Reszka, AP
Wood, AA
Read, MA
Gowan, SM
Dosanjh, HS
Trent, JO
Jenkins, TC
Kelland, LR
Neidle, S
[J]. JOURNAL OF MEDICINAL CHEMISTRY, 1998, 41 (24) : 4873 - 4884
[30] Differential sensitivity of human mammary epithelial and breast carcinoma cell lines to curcumin
Ramachandran, C
You, W
[J]. BREAST CANCER RESEARCH AND TREATMENT, 1999, 54 (03) : 269 - 278

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