NRF-2 transcription factor is essential in promoting human Tomm70 gene expression

被引：22

作者：

Blesa, JR ^{[1
]}

Hernández, JM ^{[1
]}

Hernández-Yago, J ^{[1
]}

机构：

[1] Fundac Valenciana Invest Biomed, Inst Invest Citol, Valencia 46010, Spain

来源：

MITOCHONDRION | 2004年 / 3卷 / 05期

关键词：

human Tomm70 gene; NRF-2 transcription factor; mitochondrial protein transport;

D O I：

10.1016/j.mito.2004.02.001

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

TOMM70 is a subunit of the outer mitochondrial membrane translocase that plays a major role as a receptor of hydrophobic pre-proteins targeted to the mitochondria. We report the presence of two nuclear respiratory factor 2 (NRF-2) binding motifs in the 5'-flanking region of the human Tomm70 gene and establish their essential role for promoter activity by using reporter assays in HeLa cells. We show that both NRF-2 binding sites are functional and present evidence that interactions between these sites and a CpG island contribute to expression. Mobility shift assays show that these NRF-2 sites are specifically recognized by NRF-2 present in HeLa nuclear extracts. (C) 2004 Elsevier B.V. and Mitochondria Research Society. All rights reserved.

引用

页码：251 / 259

页数：9

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