Amperometric biosensor with physically immobilized glucose oxidase on a PVA cryogel membrane

被引：28

作者：

Doretti, L ^{[1
]}

Ferrara, D ^{[1
]}

Gattolin, P ^{[1
]}

Lora, S ^{[1
]}

机构：

[1] CNR,IST FOTOCHIM & RADIAZ ALTA ENERGIA,I-35020 LEGNARO,PADOVA,ITALY

来源：

TALANTA | 1997年 / 44卷 / 05期

关键词：

biosensors glucose oxidase; physical immobilization; PVA cryogels;

D O I：

10.1016/S0039-9140(96)02130-3

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new method of physically immobilizing a biomolecule of analytical interest in poly(vinyl alcohol) cryogels was developed to obtain suitable biosensors. An amperometric glucose sensor was constructed using glucose oxidase immobilized on membranes obtained by a freezing-thawing cyclic process; No chemical cross-linking agent was used. Sensor behaviour was evaluated electrochemically with a hydrogen peroxide electrode. The glucose content in standard solutions was determined and linear calibration curves in the 5 x 10(-5)-3 x 10(-3) mol l(-1) range were obtained. Temperature and pH effects on the electrochemical response were described and kinetic parameters in the immobilized system were evaluated. (C) 1997 Elsevier Science B.V.

引用

页码：859 / 866

页数：8

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