Unique community structure and biogeography of soybean rhizobia in the saline-alkaline soils of Xinjiang, China

被引:91
作者
Han, Li Li [1 ]
Wang, En Tao [1 ,2 ]
Han, Tian Xu [1 ,4 ]
Liu, Jie [3 ]
Sui, Xin Hua [1 ]
Chen, Wen Feng [1 ]
Chen, Wen Xin [1 ]
机构
[1] China Agr Univ, Coll Biol Sci, State Key Labs Agro Biotechnol, Beijing 100094, Peoples R China
[2] Inst Politecn Nacl, Dept Microbiol, Escuela Nacl Ciencias Biol, Mexico City 11340, DF, Mexico
[3] Qingdao Univ Sci & Technol, Coll Chem Engn, Dept Biol & Pharmaceut Engn, Qingdao 266042, Peoples R China
[4] Natl Inst Biol Sci, Beijing 102206, Peoples R China
基金
中国国家自然科学基金;
关键词
Biogeography; Phylogeny; Symbiosis; Soybean; Rhizobia; Xinjiang; MULTILOCUS SEQUENCE-ANALYSIS; GENETIC DIVERSITY; BRADYRHIZOBIUM-JAPONICUM; EVOLUTIONARY GENETICS; NATURAL-POPULATIONS; GALEGA-ORIENTALIS; INOCULANT STRAIN; GLYCINE-MAX; SP-NOV; IDENTIFICATION;
D O I
10.1007/s11104-009-9956-6
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
To investigate the community composition and biogeography of soybean rhizobia in Xinjiang, a total of 151 strains were investigated with RFLP and phylogenetic analyses of 16S rRNA gene, 16S-23S intergenic spacer, three housekeeping genes (atpD, glnII and recA), and two symbiotic genes (nifH and nodC), as well as cross-nodulation. Two rhizobial species, Bradyrhizobium liaoningense and Sinorhizobium fredii, were found as dominant groups in communities of soybean rhizobia in Xinjiang, whereas three Rhizobium genomic species, B. yuanmingense and B. japonicum, were minor groups. These genomic species showed clear correlations with eco-regions, and their symbiotic genes were identical or very similar to those of the reference strains for the corresponding species. Conclusively, the dominant soybean rhizobia S. fredii and B. liaoningense in Xinjiang might be introduced from other Chinese regions, but they have been selected as the rhizobia adapted to the saline-alkaline soils. The high pH, salinity, and phosphate concentration in soil might be the environmental factors determining the biogeography of these bacteria. It is worth mentioning that a novel Rhizobium species that may have acquired the symbiotic genes from a Bradyrhizobium lineage was identified.
引用
收藏
页码:291 / 305
页数:15
相关论文
共 47 条
[1]   Comparison of molecular and antibiotiic resistance profile methods for the population analysis of Bradyrhizobium spp. (TGx) isolates that nodulate the new TGx soybean cultivars in Africa [J].
Abaidoo, RC ;
Keyser, HH ;
Singleton, PW ;
Borthakur, D .
JOURNAL OF APPLIED MICROBIOLOGY, 2002, 92 (01) :109-117
[2]   Phylogenetic analyses of Bradyrhizobium strains nodulating soybean (Glycine max) in Thailand with reference to the USDA strains of Bradyrhizobium [J].
Ando, S ;
Yokoyama, T .
CANADIAN JOURNAL OF MICROBIOLOGY, 1999, 45 (08) :639-645
[3]   Symbiotic and genetic diversity of Rhizobium galegae isolates collected from the Galega orientalis gene center in the Caucasus [J].
Andronov, EE ;
Terefework, Z ;
Roumiantseva, ML ;
Dzyubenko, NI ;
Onichtchouk, OP ;
Kurchak, ON ;
Dresler-Nurmi, A ;
Young, JPW ;
Simarov, BV ;
Lindström, K .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (02) :1067-1074
[4]  
[Anonymous], 1970, MANUAL PRACTICAL STU
[5]   Genetic diversity of native bradyrhizobia isolated from soybeans (Glycine max L.) in different agricultural-ecological-climatic regions of India [J].
Appunu, Chinnaswamy ;
N'Zoue, Angele ;
Laguerre, Gisele .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2008, 74 (19) :5991-5996
[6]   Evidence of horizontal transfer of symbiotic genes from a Bradyrhizobium japonicum inoculant strain to indigenous diazotrophs Sinorhizobium (Ensifer) fredii and Bradyrhizobium elkanii in a Brazilian Savannah soil [J].
Barcellos, Fernando Gomes ;
Menna, Pamela ;
da Silva Batista, Jesiane Stefania ;
Hungria, Mariangela .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2007, 73 (08) :2635-2643
[7]   Effect of soil bradyrhizobia on the success of soybean inoculant strain CB 1809 [J].
Botha, WJ ;
Jaftha, JB ;
Bloem, JF ;
Habig, JH ;
Law, IJ .
MICROBIOLOGICAL RESEARCH, 2004, 159 (03) :219-231
[8]   Soils of the Chinese Hubei province show a very high diversity of Sinorhizobium fredii strains [J].
Camacho, M ;
Santamaría, C ;
Temprano, E ;
Rodríguez-Navarro, DN ;
Daza, A ;
Espuny, R ;
Bellogín, R ;
Ollero, FJ ;
Lyrade, MCCP ;
Buendía-Clavería, A ;
Zhou, J ;
Li, FD ;
Mateos, C ;
Velázquez, E ;
Vinardell, JM ;
Ruiz-Sainz, JE .
SYSTEMATIC AND APPLIED MICROBIOLOGY, 2002, 25 (04) :592-602
[9]   Genetic characterization of soybean rhizobia in Paraguay? [J].
Chen, LS ;
Figueredo, A ;
Pedrosa, FO ;
Hungria, M .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (11) :5099-+
[10]   CHARACTERISTICS OF RHIZOBIUM-TIANSHANENSE SP-NOV, A MODERATELY AND SLOWLY GROWING ROOT-NODULE BACTERIUM ISOLATED FROM AN ARID SALINE ENVIRONMENT IN XINJIANG, PEOPLES-REPUBLIC-OF-CHINA [J].
CHEN, WX ;
WANG, E ;
WANG, SY ;
LI, YB ;
CHEN, XQ ;
LI, YB .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1995, 45 (01) :153-159