Development of a class-specific monoclonal antibody-based ELISA for aflatoxins in peanut

被引：86

作者：

Li, Peiwu ^{[1
]}

Zhang, Qi ^{[1
]}

Zhang, Wen ^{[1
]}

Zhang, Jinyang ^{[1
]}

Chen, Xiaomei ^{[1
]}

Jiang, Jun ^{[1
]}

Xie, Lihua ^{[1
]}

Zhang, Daohong ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Oil Crops Res Inst, Dept Qual Standard & Food Safety, Wuhan 430062, Peoples R China

来源：

FOOD CHEMISTRY | 2009年 / 115卷 / 01期

关键词：

Aflatoxins; Class-specific; Monoclonal antibody; Enzyme-linked immunosorbent assay (ELISA); FLUORESCENCE DETECTION; AFFINITY; IMMUNOASSAY; PRODUCTS; MILK; FOOD; B-1;

D O I：

10.1016/j.foodchem.2008.11.052

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Three class-specific monoclonal antibodies against aflatoxins were screened by a designed strategy in which aflatoxin G(2) was used as competitor in the screening ELISA system. With a high cross-reactivity (65%) to aflatoxin G(2), antibody 10C9 had the most similar sensitivity for five aflatoxins (AFB(1), AFB(2), AFG(1), AFG(2) and AFM(1)), whose I-50 values were in a range of 2.1-3.2 ng ml (1). So, antibody 10C9 was selected to develop an ELISA for determination of aflatoxin B-1, B-2, G(1), G(2) and total of them in peanut samples. And spiked recoveries were from 87.5% to 102.0%. The results indicate that the ELISA developed can accurately determine total aflatoxins in samples of peanuts after the simple and rapid extraction procedure. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：313 / 317

页数：5

共 30 条

[1]

[Anonymous], 1994, TOXICOLOGY AFLATOXIN

[2]

[Anonymous], 1998, Official Journal European Community L, V201, P43

[3] Determination of aflatoxin levels in some dairy and food products which consumed in Ankara, Turkey [J].

Aycicek, H ;

Aksoy, A ;

Saygi, S .

FOOD CONTROL, 2005, 16 (03) :263-266

[4] MEASUREMENT OF MONOCLONAL-ANTIBODY AFFINITY BY NONCOMPETITIVE ENZYME-IMMUNOASSAY [J].

BEATTY, JD ;

BEATTY, BG ;

VLAHOS, WG .

JOURNAL OF IMMUNOLOGICAL METHODS, 1987, 100 (1-2) :173-179

[5] Development and integration of an immunoaffinity monolithic disk for the on-line solid-phase extraction and HPLC determination with fluorescence detection of aflatoxin B1 in aqueous solutions [J].

Calleri, E. ;

Marrubini, G. ;

Brusotti, G. ;

Massolini, G. ;

Caccialanza, G. .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2007, 44 (02) :396-403

[6] AFLATOXIN MONOCLONALS - ACADEMIC DEVELOPMENT TO COMMERCIAL PRODUCTION [J].

CANDLISH, AAG ;

SMITH, JE ;

STIMSON, WH .

LETTERS IN APPLIED MICROBIOLOGY, 1990, 10 (04) :167-169

[7] Comparison of hybridoma screening methods for the efficient detection of high-affinity hapten-specific monoclonal antibodies [J].

Cervino, Christian ;

Weber, Ekkehard ;

Knopp, Dietmar ;

Niessner, Reinhard .

JOURNAL OF IMMUNOLOGICAL METHODS, 2008, 329 (1-2) :184-193

[8]

*COD AL COMM, 2001, 33 SESS CODEX HAG NE

[9] Production and characterization of monoclonal antibodies for aflatoxin B1 [J].

Devi, KT ;

Mayo, MA ;

Reddy, KLN ;

Delfosse, P ;

Reddy, G ;

Reddy, SV ;

Reddy, DVR .

LETTERS IN APPLIED MICROBIOLOGY, 1999, 29 (05) :284-288

[10] Aflatoxin-producing Aspergillus species from Thailand [J].

Ehrlich, Kenneth C. ;

Kobbeman, Kerri ;

Montalbano, Beverly G. ;

Cotty, Peter J. .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2007, 114 (02) :153-159

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