Effects of cholecystokinin and bombesin on the expression of preprosomatostatin-encoding genes in goldfish forebrain

被引:21
作者
Canosa, LF [1 ]
Peter, RE [1 ]
机构
[1] Univ Alberta, Dept Biol Sci, Edmonton, AB T6G 2E9, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
SRIF; teleost fish; GRP; growth hormone; food intake;
D O I
10.1016/j.regpep.2004.04.011
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
It was previously demonstrated that both cholecystokinin (CCK) and bombesin (BBS) stimulate growth hormone (GH) secretion in goldfish. Both peptides induce satiety and it was speculated that they integrate satiation and the postprandial increase in GH circulating levels. In the present paper we investigated the effects of CCK and BBS on the forebrain expression of the somatostatin gene family in goldfish to analyze if somatostatin peptides may be part of the effector mechanisms of CCK and BBS. We found that peripherally as well as centrally administered CCK decreases mRNA levels of preprosomatostatin (PSS)-I that encodes for SRIF-14, having no effects on PSS-II and PSS-III, which encode for gSRIF-28 and [Pro(2)] SRIF-14, respectively. In addition, a direct action on the pituitary to stimulate GH release, this inhibition of PSS-I expression provides a possible mechanism for CCK to increase postprandial GH levels. On the other hand, BBS inhibits the forebrain expression of PSS-I and PSS-II but does not affect PSS-III regardless of the route of administration. We conclude that this could be the most likely mechanism of action of BBS to increase GH secretion, since there are few BBS-immunoreactive (IR) fibers and BBS binding sites in the anterior pituitary of goldfish. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:99 / 105
页数:7
相关论文
共 53 条
[31]  
Jensen J., 1994, COMP PHYSL EVOLUTION, V199, P119
[32]   Phylogeny of the cholecystokinin/gastrin family [J].
Johnsen, AH .
FRONTIERS IN NEUROENDOCRINOLOGY, 1998, 19 (02) :73-99
[33]   GROWTH-HORMONE INCREASES GROWTH-RATE, APPETITE AND DOMINANCE IN JUVENILE RAINBOW-TROUT, ONCORHYNCHUS-MYKISS [J].
JOHNSSON, JI ;
BJORNSSON, BT .
ANIMAL BEHAVIOUR, 1994, 48 (01) :177-186
[34]   Ghrelin is a growth-hormone-releasing acylated peptide from stomach [J].
Kojima, M ;
Hosoda, H ;
Date, Y ;
Nakazato, M ;
Matsuo, H ;
Kangawa, K .
NATURE, 1999, 402 (6762) :656-660
[35]   Expression of three distinct somatostatin messenger ribonucleic acids (mRNAs) in goldfish brain: Characterization of the complementary deoxyribonucleic acids, distribution and seasonal variation of the mRNAs, and action of a somatostatin-14 variant [J].
Lin, XW ;
Otto, CJ ;
Peter, RE .
ENDOCRINOLOGY, 1999, 140 (05) :2089-2099
[36]   Brain regulation of feeding behavior and food intake in fish [J].
Lin, XW ;
Volkoff, H ;
Narnaware, Y ;
Bernier, NJ ;
Peyon, P ;
Peter, RE .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 2000, 126 (04) :415-434
[37]   RELATIONSHIP BETWEEN SERUM GROWTH-HORMONE LEVELS AND THE BRAIN AND PITUITARY CONTENT OF IMMUNOREACTIVE SOMATOSTATIN IN THE GOLDFISH, CARASSIUS-AURATUS L [J].
MARCHANT, TA ;
DULKA, JG ;
PETER, RE .
GENERAL AND COMPARATIVE ENDOCRINOLOGY, 1989, 73 (03) :458-468
[38]   BOMBESIN - POTENTIAL INTEGRATIVE PEPTIDE FOR FEEDING AND SATIETY [J].
MCCOY, JG ;
AVERY, DD .
PEPTIDES, 1990, 11 (03) :595-607
[39]   CHARACTERIZATION OF A GASTRIN RELEASING PEPTIDE FROM PORCINE NON-ANTRAL GASTRIC TISSUE [J].
MCDONALD, TJ ;
JORNVALL, H ;
NILSSON, G ;
VAGNE, M ;
GHATEI, M ;
BLOOM, SR ;
MUTT, V .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1979, 90 (01) :227-233
[40]   COMPARATIVE DISTRIBUTION OF SUBSTANCE-P (SP) AND CHOLECYSTOKININ (CCK) BINDING-SITES AND IMMUNOREACTIVITY IN THE BRAIN OF THE SEA BASS (DICENTRARCHUS-LABRAX) [J].
MOONS, L ;
BATTEN, TFC ;
VANDESANDE, F .
PEPTIDES, 1992, 13 (01) :37-46