Automated formation of lipid-bilayer membranes in a microfluidic device

被引：161

作者：

Malmstadt, Noah

Nash, Michael A.

Purnell, Robert F.

Schmidt, Jacob J. ^{[1
]}

机构：

[1] Univ Calif Los Angeles, Dept Bioengn, Los Angeles, CA 90095 USA

[2] Univ Calif Los Angeles, Cybernet Interdept Program, Los Angeles, CA 90095 USA

[3] Univ Calif Los Angeles, Calif Nanosyst Inst, Los Angeles, CA 90095 USA

来源：

NANO LETTERS | 2006年 / 6卷 / 09期

关键词：

D O I：

10.1021/nl0611034

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Although membrane channel proteins are important to drug discovery and hold great promise as engineered nanopore sensing elements, their widespread application to these areas has been limited by difficulties in fabricating planar lipid-bilayer membranes. We present a method for forming these sub-5-nm-thick free-standing structures based on a self-assembly process driven by solvent extraction in a microfluidic channel. This facile automatable process forms high-quality membranes able to host channel proteins measurable at single-molecule conductance resolution.

引用

收藏

页码：1961 / 1965

页数：5

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