Infrared atmospheric pressure MALDI ion trap mass Spectrometry of frozen samples using a peltier-cooled sample stage

被引：17

作者：

Von Seggern, CE ^{[1
]}

Gardner, BD ^{[1
]}

Cotter, RJ ^{[1
]}

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Pharmacol & Mol Sci, Baltimore, MD 21205 USA

来源：

ANALYTICAL CHEMISTRY | 2004年 / 76卷 / 19期

关键词：

D O I：

10.1021/ac049575v

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Infrared atmospheric pressure matrix-assisted laser desorption/ionization on an ion trap mass spectrometer is used to analyze frozen samples generated using a Peltier-cooled sample stage. This allows for the analysis of samples in water without the addition of matrix, in near-native conditions, and with minimal loss of water due to evaporation. Analysis of frozen samples is extended to study peptides, carbohydrates, and glycolipids.

引用

页码：5887 / 5893

页数：7

共 25 条

[1] INFRARED LASER-INDUCED DESORPTION OF NEUTRAL ORGANIC-COMPOUNDS FROM FROZEN AQUEOUS-SOLUTION FOLLOWED BY SINGLE-PHOTON IONIZATION [J].

BECKER, CH ;

JUSINSKI, LE ;

MORO, L .

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 1990, 95 (03) :R1-R4

[2] Ice as a matrix for IR-matrix-assisted laser desorption/ionization: Mass spectra from a protein single crystal [J].

Berkenkamp, S ;

Karas, M ;

Hillenkamp, F .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (14) :7003-7007

[3] Laser desorption and imaging of proteins from ice via UV femtosecond laser pulses [J].

Berry, JI ;

Sun, SX ;

Dou, YS ;

Wucher, A ;

Winograd, N .

ANALYTICAL CHEMISTRY, 2003, 75 (19) :5146-5151

[4] Structural analysis of oligosaccharides by atmospheric pressure matrix-assisted laser desorption/ionisation quadrupole ion trap mass spectrometry [J].

Creaser, CS ;

Reynolds, JC ;

Harvey, DJ .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2002, 16 (03) :176-184

[5]

DANELL RA, 2000, P 48 ASMS C MASS SPE

[6] A SYSTEMATIC NOMENCLATURE FOR CARBOHYDRATE FRAGMENTATIONS IN FAB-MS MS SPECTRA OF GLYCOCONJUGATES [J].

DOMON, B ;

COSTELLO, CE .

GLYCOCONJUGATE JOURNAL, 1988, 5 (04) :397-409

[7] Atmospheric pressure matrix-assisted laser desorption/ionization in transmission geometry [J].

Galicia, MC ;

Vertes, A ;

Callahan, JH .

ANALYTICAL CHEMISTRY, 2002, 74 (08) :1891-1895

[8] Cryogenic frozen solution matrixes for analysis of DNA by time-of-flight mass spectrometry [J].

Hunter, JM ;

Lin, HA ;

Becker, CH .

ANALYTICAL CHEMISTRY, 1997, 69 (17) :3608-3612

[9] Infrared, surface-assisted laser desorption ionization mass spectrometry on frozen aqueous solutions of proteins and peptides using suspensions of organic solids [J].

Kraft, P ;

Alimpiev, S ;

Dratz, E ;

Sunner, J .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1998, 9 (09) :912-924

[10] Atmospheric pressure MALDI/ion trap mass spectrometry [J].

Laiko, VV ;

Moyer, SC ;

Cotter, RJ .

ANALYTICAL CHEMISTRY, 2000, 72 (21) :5239-5243

← 1 2 3 →