Production of inulinase from Kluyveromyces marxianus YS-1 using root extract of Asparagus racemosus

被引：58

作者：

Singh, R. S. ^{[1
]}

Dhaliwal, Rajesh ^{[1
]}

Puri, Munish ^{[1
]}

机构：

[1] Punjabi Univ, Dept Biotechnol, Carbohydrate & Prot Biotechnol Lab, Patiala 147002, Punjab, India

来源：

PROCESS BIOCHEMISTRY | 2006年 / 41卷 / 07期

关键词：

Kluyveromyces marxianus YS-1; Asparagus racemosus; root extract; inulinase; production; optimization;

D O I：

10.1016/j.procbio.2006.03.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Roots of Asparagus racemosus were used as a source of inulin for the production of inulinase from Kluyveromyces marxianus YS-1. Root extract prepared at 10 kg/cm(2) for 10 min showed the maximum production of inulinase. Various parameters of fermentation have been examined in order to improve overall enzyme yield. Inulinase yield of 40.5 IU/mL in an optimized medium containing inulin (3.5%), beef extract (0.5%), MnSO4 (0.5 MW CoCl2 (0.05 mM), SDS (0.4 mM) and pH 6.5 at 30 degrees C under agitation (150 rpm) for 60 h has been obtained at shake flask level. After optimization the enzyme production has been increased 6.1 times at flask level. In a bioreactor (working volume I L) fermentation medium containing 4% inulin has shown maximum inulinase production (47.3 IU/mL) under optimized parameters of 200 rpm, 0.75 vvm aeration at 30 degrees C after 60 h. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：1703 / 1707

页数：5

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