Genetic selection strategies for generating and characterizing catalysts

被引：12

作者：

Kast, P ^{[1
]}

Hilvert, D ^{[1
]}

机构：

[1] SCRIPPS RES INST, DEPT MOL BIOL, LA JOLLA, CA 92037 USA

来源：

PURE AND APPLIED CHEMISTRY | 1996年 / 68卷 / 11期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1351/pac199668112017

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Molecular evolution is a powerful tool for exploring and creating catalytic function in biological macromolecules. This review focuses on examples and current strategies for the application of random mutagenesis of target genes coupled with direct selection in vivo. The technology is illustrated by the dissection of catalytic features in chorismate mutase. Future avenues for identifying and evolving catalytic antibodies are also discussed.

引用

页码：2017 / 2024

页数：8

共 102 条

[11] INVENTING AND IMPROVING RIBOZYME FUNCTION - RATIONAL DESIGN VERSUS ITERATIVE SELECTION METHODS
BREAKER, RR
JOYCE, GF
[J]. TRENDS IN BIOTECHNOLOGY, 1994, 12 (07) : 268 - 275
[12] SELF-INCORPORATION OF COENZYMES BY RIBOZYMES
BREAKER, RR
JOYCE, GF
[J]. JOURNAL OF MOLECULAR EVOLUTION, 1995, 40 (06) : 551 - 558
[13] SYNTHETIC RIBOZYMES AND THE FIRST DEOXYRIBOZYME
BURGSTALLER, P
FAMULOK, M
[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH, 1995, 34 (11): : 1189 - 1192
[14] ISOLATION OF RNA APTAMERS FOR BIOLOGICAL COFACTORS BY IN-VITRO SELECTION
BURGSTALLER, P
FAMULOK, M
[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH, 1994, 33 (10): : 1084 - 1087
[15] CADWELL RC, 1994, PCR METH APPL, V3, pS136
[16] IN-VITRO SELECTION OF CATALYTIC RNAS
CHAPMAN, KB
SZOSTAK, JW
[J]. CURRENT OPINION IN STRUCTURAL BIOLOGY, 1994, 4 (04) : 618 - 622
[17] CRYSTAL-STRUCTURES OF THE MONOFUNCTIONAL CHORISMATE MUTASE FROM BACILLUS-SUBTILIS AND ITS COMPLEX WITH A TRANSITION-STATE ANALOG
CHOOK, YM
KE, HM
LIPSCOMB, WN
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (18) : 8600 - 8603
[18] THE MONOFUNCTIONAL CHORISMATE MUTASE FROM BACILLUS-SUBTILIS - STRUCTURE DETERMINATION OF CHORISMATE MUTASE AND ITS COMPLEXES WITH A TRANSITION-STATE ANALOG AND PREPHENATE, AND IMPLICATIONS FOR THE MECHANISM OF THE ENZYMATIC-REACTION
CHOOK, YM
GRAY, JV
KE, HM
LIPSCOMB, WN
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1994, 240 (05) : 476 - 500
[19] MAKING ANTIBODY FRAGMENTS USING PHAGE DISPLAY LIBRARIES
CLACKSON, T
HOOGENBOOM, HR
GRIFFITHS, AD
WINTER, G
[J]. NATURE, 1991, 352 (6336) : 624 - 628
[20] Mutagenesis study of active site residues in chorismate mutase from Bacillus subtilis
Cload, ST
Liu, DR
Pastor, RM
Schultz, PG
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1996, 118 (07) : 1787 - 1788

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