Stimuli-responsive command polymer surface for generation of protein gradients

被引：32

作者：

Ionov, Leonid ^{[1
]}

Houbenov, Nikolay ^{[2
]}

Sidorenko, Alexander ^{[3
]}

Stamm, Manfred ^{[2
]}

Minko, Sergiy ^{[4
]}

机构：

[1] Max Planck Inst Mol Cell Biol & Genet, D-01307 Dresden, Germany

[2] Leibniz Inst Polymer Res Dresden eV, D-01069 Dresden, Germany

[3] Univ Sci Philadelphia, Dept Chem & Biochem, Philadelphia, PA 19104 USA

[4] Clarkson Univ, NABLAB, Dept Chem & Biomol Sci, Potsdam, NY 13699 USA

来源：

BIOINTERPHASES | 2009年 / 4卷 / 02期

关键词：

adsorption; biochemistry; molecular biophysics; polymer electrolytes; polymers; proteins; FIELD LATTICE THEORY; POLYELECTROLYTE BRUSHES; ANCHORING LAYER; CELL-ADHESION; BIOMATERIALS; POLYSTYRENE; ADSORPTION; CHARGE; MULTILAYERS; FABRICATION;

D O I：

10.1116/1.3119722

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Mixed polyelectrolyte brushes with a composition gradient were used as a platform for fabrication of stimuli-responsive command surfaces to control the generation of concentration gradients of adsorbed protein molecules. Switching between homogeneously adsorbed protein layers and adsorbed layers with protein concentration gradients was achieved by changing the pH of protein aqueous solutions. Protein adsorption and the direction of the adsorption gradient were tuned and also turned off and on or reversed by tuning the proton concentration in the pH range 4.0-8.6.

引用

页码：FA45 / FA49

页数：5

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