Identification of Vitis vinifera (-)-α-terpineol synthase by in silico screening of full-length cDNA ESTs and functional characterization of recombinant terpene synthase

被引：119

作者：

Martin, DM ^{[1
]}

Bohlmann, J ^{[1
]}

机构：

[1] Univ British Columbia, Biotechnol Lab, Vancouver, BC V6T 1Z3, Canada

来源：

PHYTOCHEMISTRY | 2004年 / 65卷 / 09期

基金：

加拿大自然科学与工程研究理事会; 加拿大创新基金会;

关键词：

Vitis vinifera; vitaceae; grape vine; wine flavour and aroma; terpene cyclase;

D O I：

10.1016/j.phytochem.2004.03.018

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The flavour and aroma of certain Vitis vinifera grape varieties is dominated by volatile terpenes and small volatile aldehydes. Monoterpenes contribute to the final grape and wine aroma and flavour in form of free volatiles and as glycoside conjugates of monoterpene alcohols. Typical monoterpenol components of the cultivar Gewurztraminer and other aroma-rich grape varieties are linalool, geraniol, nerol, citronellol, and alpha-terpineol. In a functional genomics effort to identify genes for the formation of monoterpene alcohols in V. vinifera, a database of full-length cDNA sequences was screened in silico and yielded two clones for putative monoterpene synthases. The gene products were functionally characterized by expression in Escherichia coli, in vitro enzyme assay and gas chromatography-mass spectrometry (GC-MS) product identification as multi-product (-)-alpha-terpineol synthases. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：1223 / 1229

页数：7

共 28 条

[1]

BASIC LOCAL ALIGNMENT SEARCH TOOL [J].

ALTSCHUL, SF ;

GISH, W ;

MILLER, W ;

MYERS, EW ;

LIPMAN, DJ .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410

[2]

Genomic analysis of the terpenoid synthase (AtTPS) gene family of Arabidopsis thaliana [J].

Aubourg, S ;

Lecharny, A ;

Bohlmann, J .

MOLECULAR GENETICS AND GENOMICS, 2002, 267 (06) :730-745

[3]

Plant terpenoid synthases: Molecular biology and phylogenetic analysis [J].

Bohlmann, J ;

Meyer-Gauen, G ;

Croteau, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (08) :4126-4133

[4]

Burke YD, 2002, ANTICANCER RES, V22, P3127

[5]

Cane D. E., 1999, Comprehensive Natural Products Chemistry, P155, DOI [DOI 10.1016/B978-0-08-091283-7.00039-4, 10.1016/B978-0-08-091283-7.00039-4]

[6]

PURIFICATION AND CHARACTERIZATION OF GERANYL DIPHOSPHATE SYNTHASE FROM VITIS-VINIFERA L CV MUSCAT DE FRONTIGNAN CELL-CULTURES [J].

CLASTRE, M ;

BANTIGNIES, B ;

FERON, G ;

SOLER, E ;

AMBID, C .

PLANT PHYSIOLOGY, 1993, 102 (01) :205-211

[7]

Croteau R., 2000, Biochemistry Molecular Biology of Plants, P1250, DOI DOI 10.1201/B11003-3

[8]

Prevention and therapy of cancer by dietary monoterpenes [J].

Crowell, PL .

JOURNAL OF NUTRITION, 1999, 129 (03) :775S-778S

[9]

Towards the transcriptome of grapevine (Vitis vinifera L.) [J].

Driesel, AJ ;

Lommele, Å ;

Drescher, B ;

Töpfer, R ;

Bell, M ;

Cartharius, I ;

Cheutin, N ;

Huck, JF ;

Kubiak, J ;

Regnard, P ;

Steinmetz, A .

PROCEEDINGS OF THE 8TH INTERNATIONAL CONFERENCE ON GRAPE GENETICS AND BREEDING, VOLS 1 AND 2, 2003, (603) :239-249

[10]

(E)-β-ocimene and myrcene synthase genes of floral scent biosynthesis in snapdragon:: Function and expression of three terpene synthase genes of a new terpene synthase subfamily [J].

Dudareva, N ;

Martin, D ;

Kish, CM ;

Kolosova, N ;

Gorenstein, N ;

Fäldt, J ;

Miller, B ;

Bohlmann, J .

PLANT CELL, 2003, 15 (05) :1227-1241

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