The GluR2 subunit inhibits proliferation by inactivating Src-MAPK signalling and induces apoptosis by means of caspase 3/6-dependent activation in glioma cells

被引：32

作者：

Beretta, Francesca ^{[1
,2
]}

Bassani, Silvia ^{[2
]}

Binda, Elena ^{[3
]}

Verpelli, Chiara ^{[2
,4
]}

Bello, Lorenzo ^{[4
]}

Galli, Rossella ^{[3
]}

Passafaro, Maria ^{[1
,2
]}

机构：

[1] DTI Dulbecco Telethon Inst, I-20129 Milan, Italy

[2] Univ Milan, Dept Pharmacol, CNR, Inst Neurosci Cellular & Mol Pharmacol, I-20122 Milan, Italy

[3] Univ Milan, Dept Pharmacol, CNR, Inst Neurosci, I-20129 Milan, Italy

[4] Univ Milan, Neurosurg Unit, Fdn Ist Ricovero & Cura Carattere Sci, Osped Maggiore Policlin, I-20129 Milan, Italy

来源：

EUROPEAN JOURNAL OF NEUROSCIENCE | 2009年 / 30卷 / 01期

关键词：

ERK1; 2; glioma specimens; GRIP; tumour stem cells; AMPA RECEPTOR SUBUNITS; HUMAN GLIOBLASTOMA; HIPPOCAMPAL-NEURONS; KINASE; PROTEIN; GROWTH; EXPRESSION; INTERACTS; NECROSIS; PATHWAY;

D O I：

10.1111/j.1460-9568.2009.06804.x

中图分类号：

Q189 [神经科学];

学科分类号：

071006 [神经生物学];

摘要：

Glioblastoma multiforme (GBM) is the most invasive and undifferentiated type of brain tumour, and so surgical interventions are ineffective. We found that GluR2 is absent in fast-growing GBM-derived tumour stem cells and high-grade glioma specimens, but is expressed in slow-growing stem cells and low-grade glioma specimens. More remarkably, GluR2 overexpression in U-87MG cells inhibits proliferation by inactivating extracellular signal-regulated kinase (ERK)1/2-Src phosphorylation and induces apoptosis. Mechanistically, we observed that the scaffold protein GRIP is essential for the effect of GluR2 on ERK-Src inactivation. These findings indicate that the absence of the GluR2 subunit favours malignancy.

引用

页码：25 / 34

页数：10

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