Metabolic engineering of Saccharomyces cerevisiae

被引：275

作者：

Ostergaard, S ^{[1
]}

Olsson, L ^{[1
]}

Nielsen, J ^{[1
]}

机构：

[1] Tech Univ Denmark, Dept Biotechnol, Ctr Proc Biotechnol, DK-2800 Lyngby, Denmark

来源：

MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS | 2000年 / 64卷 / 01期

关键词：

D O I：

10.1128/MMBR.64.1.34-50.2000

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Comprehensive knowledge regarding Saccharomyces cerevisiae has accumulated over time, and today S. cerevisiae serves as a widley used biotechnological production organism as well as a eukaryotic model system. The high transformation efficiency, in addition to the availability of the the complete yeast genome sequence has facilitated genetic manipulation of this microorganism, and new approaches are constantly being taken to metabolicially engineer this organism in oder to suit specific needs. In this paper; strategies and concepts for metabolic engineering are discussed and several examples based upon selected studies involving S, cerevisiae are reviewed. The many different studies of metabolic engineering using this organism illustrate all the categories of this multidisciplinary field: extension of substrate range, improvements of producitivity and yield, elimination of byproduct formation, improvement of process performance, improvements of cellular properties, and extension of product range including heterologous protein production.

引用

页码：34 / +

页数：19

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