Association of nucleoid proteins with coding and non-coding segments of the Escherichia coli genome

被引:224
作者
Grainger, David C. [1 ]
Hurd, Douglas
Goldberg, Martin D.
Busby, Stephen J. W.
机构
[1] Univ Birmingham, Sch Biosci, Birmingham B15 2TT, W Midlands, England
[2] Oxford Gene Technol, Oxford OX5 1PF, England
基金
英国惠康基金;
关键词
D O I
10.1093/nar/gkl542
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The Escherichia coli chromosome is condensed into an ill-defined structure known as the nucleoid. Nucleoid-associated DNA-binding proteins are involved in maintaining this structure and in mediating chromosome compaction. We have exploited chromatin immunoprecipitation and high-density microarrays to study the binding of three such proteins, FIS, H-NS and IHF, across the E.coli genome in vivo. Our results show that the distribution of these proteins is biased to intergenic parts of the genome, and that the binding profiles overlap. Hence some targets are associated with combinations of bound FIS, H-NS and IHF. In addition, many regions associated with FIS and H-NS are also associated with RNA polymerase.
引用
收藏
页码:4642 / 4652
页数:11
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