Dysfunction of store-operated calcium channel in muscle cells lacking mg29

被引:145
作者
Pan, Z
Yang, DM
Nararaj, RY
Nosek, TA
Nishi, M
Takeshima, H
Cheng, HP
Ma, JJ
机构
[1] Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Dept Physiol & Biophys, Piscataway, NJ 08854 USA
[2] NIA, Cardiovasc Sci Lab, Gerontol Res Ctr, Baltimore, MD 21224 USA
[3] Peking Univ, Coll Life Sci, Natl Lab Biomembrane & Membrane Biotechnol, Beijing 100871, Peoples R China
[4] Case Western Reserve Univ, Sch Med, Dept Physiol & Biophys, Cleveland, OH 44106 USA
[5] Tohoku Univ, Grad Sch Med, Dept Biochem, Sendai, Miyagi 9808575, Japan
[6] Japan Sci & Technol Corp, CREST, Sendai, Miyagi 9808575, Japan
基金
美国国家卫生研究院;
关键词
D O I
10.1038/ncb788
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The store-operated calcium channel (SOC) located in the plasma membrane (PM) mediates capacitative entry of extracellular calcium after depletion of intracellular calcium stores in the endoplasmic or sarcoplasmic reticulum (ER/SR)(1,2). An intimate interaction between the PM and the ER/SR is essential for the operation of this calcium signalling pathway(3-5). Mitsugumin 29 (MG29) is a synaptophysin-family-related protein located in the junction between the PM and SR of skeletal muscle(6,7). Here, we identify SOC in skeletal muscle and characterise its regulation by MG29 and the ryanodine receptor (RyR) located in the SR. Targeted deletion of mg29 alters the junctional membrane structure, causes severe dysfunction of SOC and SR calcium homeostasis and increases the susceptibility of muscle to fatigue stimulation(8). Severe dysfunction of SOC is also identified in muscle cells lacking both type 1 and type 3 RyRs, indicating that SOC activation requires an intact interaction between the PM and the SR, and is linked to conformational changes of RyRs. Whereas defective SOC seems to be inconsequential to short-term excitation contraction coupling, the slow cumulative calcium entry through SOC is crucial for long-term calcium homeostasis, such that reduced SOC activity exaggerates muscle fatigue under conditions of intensive exercise.
引用
收藏
页码:379 / 383
页数:5
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