Comparison of cultures from rectoanal-junction mucosal swabs and feces for detection of Escherichia coli O157 in dairy heifers

被引:38
作者
Davis, Margaret A.
Rice, Daniel H.
Sheng, Haiqing
Hancock, Dale D.
Besser, Thomas E.
Cobbold, Rowland
Hovde, Carolyn J. [1 ]
机构
[1] Univ Idaho, Dept Microbiol Mol Biol & Biochem, Moscow, ID 83844 USA
[2] Washington State Univ, Dept Vet Clin Sci, Pullman, WA 99164 USA
[3] Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA
关键词
D O I
10.1128/AEM.72.5.3766-3770.2006
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Fecal culture for Escherichia coli O157:H7 was compared to rectoanal mucosal swab (RAMS) culture in dairy heifers over a 1-year period. RAMS enrichment culture was as sensitive as fecal culture using immunomagnetic separation (IMS) (P = 0.98, as determined by a chi-square test). RAMS culture is less costly than fecal IMS culture and can yield quantitative data.
引用
收藏
页码:3766 / 3770
页数:5
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