Import of assembled PTS1 proteins into peroxisomes of the yeast Hansenula polymorpha:: Yes and No!

被引：32

作者：

Faber, KN ^{[1
]}

van Dijk, R ^{[1
]}

Keizer-Gunnink, I ^{[1
]}

Koek, A ^{[1
]}

van der Klei, IJ ^{[1
]}

Veenhuis, M ^{[1
]}

机构：

[1] Univ Groningen, NL-9750 AA Haren, Netherlands

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 2002年 / 1591卷 / 1-3期

关键词：

Hansenula polymorpha; PTS; 1; peroxisome;

D O I：

10.1016/S0167-4889(02)00274-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previously, Waterham et al. [EMBO J. 12 (1993) 4785] reported that cytosolic oligomeric alcohol oxidase (AO) is not incorporated into peroxisomes after reassembly of the organelles in the temperature-sensitive peroxisome-deficient mutant pex1-6(ts) of Hansenula polymorpha shifted to permissive growth conditions. Here, we show that the failure to import assembled AO protein is not exemplary for other folded proteins because both an artificial peroxisomal matrix protein, PTS1-tagged GFP (GFP.SKL), and the endogenous dimeric PTS1 protein dihydroxyacetone synthase (DHAS) were imported under identical conditions. In vitro receptor-ligand binding studies using immobilised H. polymorpha Pex5p and crude extracts of methanol-induced pex1-6(ts) cells, showed that AO octamers did not interact with the recombinant PTS1 receptor, at conditions that allowed binding of folded GFP.SKL and dimeric DHAS. This shows that import of oligomeric proteins is not a universal pathway for peroxisomal matrix proteins. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：157 / 162

页数：6

共 28 条

[1] A stretch of positively charged amino acids at the N terminus of Hansenula polymorpha Pex3p is involved in incorporation of the protein into the peroxisomal membrane [J].