Exploring bioanalytical applications of assisted protein reassembly

被引:4
作者
Deo, SK [1 ]
机构
[1] Univ Kentucky, Dept Chem, Lexington, KY 40506 USA
关键词
assisted protein reassembly; protein-protein interactions; immunoassasy; reporter protein; molecular rulers;
D O I
10.1007/s00216-004-2633-y
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Reassembly of protein from its peptide fragments is a technique that can have many applications in the bioanalytical field. Typically, a reporter protein fragmented into its two peptides is employed as a label in this study. This fragments of peptide can reassemble yielding an active functional reporter. This reassembly of the protein can be assisted by non-covalently interacting peptides or proteins, which are attached to the fragmented reporter. This technique has been employed in several applications including study of protein-protein interactions, antibody screening, immunoassays, and high-throughput screening. This review focuses on different reporters employed in the study of reassembly of proteins and applications of this strategy in bioanalysis.
引用
收藏
页码:383 / 390
页数:8
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