Truncated mouse adenomatous polyposis coli reduces connexin32 content and increases matrilysin secretion from Paneth cells

被引:11
作者
Husoy, T
Olstorn, HB
Knutsen, HK
Loberg, EM
Cruciani, V
Mikalsen, SO
Goverud, IL
Alexander, J
机构
[1] Norwegian Inst Publ Hlth, Dept Food Toxicol, NO-0403 Oslo, Norway
[2] Ullevaal Univ Hosp, Dept Pathol, NO-0407 Oslo, Norway
[3] Norwegian Radium Hosp, Dept Environm & Occupat Canc, NO-0310 Oslo, Norway
关键词
adenomatous polyposis coli; connexin; Paneth cells; matrilysin; colon cancer;
D O I
10.1016/j.ejca.2004.02.024
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Heterozygous mutations in adenomatous polyposis coli (APC) is an early event in inheritable and sporadic colon cancer development. We recently found reduced connexin (Cx43) expression in intestinal cell lines with heterozygous Apc mutation. In this study we investigated Cx expression and the role of one mutated Apc allele in epithelia of multiple intestinal neoplasia (Min) mouse intestines by immunohistochemistry. Cx43 was not expressed in intestinal epithelia of Min and wild-type mice. Cx32 was specifically expressed in enterochromaffin cells in both mice types, and in Paneth cells of wild-type mice. In contrast, Min mice had nearly undetectable level of Cx32 in Paneth cells. Isolated small intestinal crypts from Min mice had markedly increased secretion of both lysozyme and matrilysin compared with wild-type mice. Absence of matrilysin in Min mice reduces adenoma development. Reduced Cx32 and increased matrilysin secretion from Paneth cells could be important to neoplastic development in the intestine. (C) 2004 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1599 / 1603
页数:5
相关论文
共 32 条
  • [21] EFFECT OF WNT-1 AND RELATED PROTEINS ON GAP JUNCTIONAL COMMUNICATION IN XENOPUS EMBRYOS
    OLSON, DJ
    CHRISTIAN, JL
    MOON, RT
    [J]. SCIENCE, 1991, 252 (5009) : 1173 - 1176
  • [22] A fish oil derived concentrate enriched in eicosapentaenoic and docosahexaenoic acid as ethyl ester suppresses the formation and growth of intestinal polyps in the Min mouse
    Paulsen, JE
    Elvsaas, IKO
    Steffensen, IL
    Alexander, J
    [J]. CARCINOGENESIS, 1997, 18 (10) : 1905 - 1910
  • [23] Apical membrane localization of the adenomatous polyposis coli tumor suppressor protein and subcellular distribution of the β-catenin destruction complex in polarized epithelial cells
    Reinacher-Schick, A
    Gumbiner, BM
    [J]. JOURNAL OF CELL BIOLOGY, 2001, 152 (03) : 491 - 502
  • [24] THE APC GENE-PRODUCT IN NORMAL AND TUMOR-CELLS
    SMITH, KJ
    JOHNSON, KA
    BRYAN, TM
    HILL, DE
    MARKOWITZ, S
    WILLSON, JKV
    PARASKEVA, C
    PETERSEN, GM
    HAMILTON, SR
    VOGELSTEIN, B
    KINZLER, KW
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (07) : 2846 - 2850
  • [25] 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine increases the numbers of tumors, cystic crypts and aberrant crypt foci in multiple intestinal neoplasia mice
    Steffensen, IL
    Paulsen, JE
    Eide, TJ
    Alexander, J
    [J]. CARCINOGENESIS, 1997, 18 (05) : 1049 - 1054
  • [26] SU LK, 1993, CANCER RES, V53, P2728
  • [27] High incidence of spontaneous and chemically induced liver tumors in mice deficient for connexin32
    Temme, A
    Buchmann, A
    Gabriel, HD
    Nelles, E
    Schwarz, M
    Willecke, K
    [J]. CURRENT BIOLOGY, 1997, 7 (09) : 713 - 716
  • [28] Dilated bile canaliculi and attenuated decrease of nerve-dependent bile secretion in connexin32-deficient mouse liver
    Temme, A
    Stümpel, F
    Söhl, G
    Rieber, EP
    Jungermann, K
    Willecke, K
    Ott, T
    [J]. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 2001, 442 (06): : 961 - 966
  • [29] Trosko James E., 1998, Frontiers in Bioscience, V3, pD208
  • [30] van der Heyden MAG, 1998, J CELL SCI, V111, P1741